ln vitro isolation of Ehrlichia ruminantium from ovine blood into lxodes scapularis (lDE8) cell cultures
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | ln vitro isolation of Ehrlichia ruminantium from ovine blood into lxodes scapularis (lDE8) cell cultures | |
| Creator | Zweygarth, E. Josemans, A. I. Steyn, H. C. | |
| Description | Four stocks of Ehrlichiar uminantium (Welgevonden, Ball3, Nonile and Blaauwkrans), the causative agent of heartwater in domestic ruminants, were isolated into lxodes capularis (lDE8) tick cells using the leukocyte fraction of the blood of infected sheep. Organisms of two of the E. ruminantrum stocks (Welgevonden and Blaauwkrans) propagated in IDEB cells were also successfully used to infect bovine endothelial cells. All stocks were successfully propagated in IDEB cells using Dulbecco's modified Eagle's medium nutrient mixture Ham F-12c ontaining 10% foetal bovine serum (FBS). The technique should be included in any attempt to isolate uncharacterized E. ruminantium stocks. | |
| Publisher | AOSIS | |
| Date | 2008-08-31 | |
| Identifier | 10.4102/ojvr.v75i2.10 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 75, No 2 (2008); 121-126 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/10/9
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