Enhanced expression of recombinant beta toxin of Clostridium perfringens type B using a commercially available Escherichia coli strain
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | Enhanced expression of recombinant beta toxin of Clostridium perfringens type B using a commercially available Escherichia coli strain | |
| Creator | Bakhshi, Fatemah Pilehchian Langroudi, Reza Imani, Bahram Golestani | |
| Description | Clostridium perfringens beta toxin is only produced by types B and C and plays an important role in many human and animal diseases, causing fatal conditions that originate in the intestines. We compared the expression of C. perfringens type B vaccine strain recombinant beta toxin gene in the Escherichia coli strains RosettaTM(DE3) and BL21(DE3). The beta toxin gene was extracted from pJETβ and ligated with pET22b(+). pET22β was transformed into E. coli strains BL21(DE3) and RosettaTM(DE3). Recombinant protein was expressed as a soluble protein after isopropyl β-D-1-thiogalactopyranoside (IPTG) induction in strain RosettaTM(DE3) but not in BL21(DE3). Expression was optimised by growing recombinant cells at 37 °C and at an induction of 0.5 mM, 1 mM, 1.5 mM IPTG. Expression was evaluated using sodium dodecyl sulfate Polyacrylamide gel electrophoresis (SDS-PAGE). The recombinant protein was purified via Ni-NTA and was analysed using western blot. We concluded that E. coli strain RosettaTM(DE3) can enhance the expression of C. perfringens recombinant beta toxin.Keywords: C. perfringens beta toxin (CPB); expression; RosettaTM; BL21 | |
| Publisher | AOSIS | |
| Date | 2016-06-30 | |
| Identifier | 10.4102/ojvr.v83i1.1136 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 83, No 1 (2016); 4 pages 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/1136/1493
https://ojvr.org/index.php/ojvr/article/view/1136/1494
https://ojvr.org/index.php/ojvr/article/view/1136/1495
https://ojvr.org/index.php/ojvr/article/view/1136/1489
|
|
ADVERTISEMENT
