The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | The fluorescent detection of Paradiplozoon sp. (Monogenea: Diplozoidae) attachment clamps' sclerites and integumental proteins : research communication | |
| Creator | Milne, S.J. Avenant-Oldewage, A. | |
| Description | In the genus Paradiplozoon, few hard structures are present therefore most of the taxonomic studies have focused on the unique ultrastructure of the sclerites. Alcohol-fixed specimens were transferred to BSA-saline for 5 min. before staining indefinitely with 5 l WGA-TRX, 5 l SYTO 9 and 5 l of Cal co fluor White M2R. Rhodamine, Fluorescein and DAPI bandpass filters on the microscope enabled selective light wavelength illumination of the three flourochromes by a mercury light source. This method provided an easy and rapid methodology to show the internal sclerites of attachment clamps. It is suitable for alcohol preserved specimens and may have additional applications in other helminth organisms. | |
| Publisher | AOSIS | |
| Date | 2006-09-13 | |
| Identifier | 10.4102/ojvr.v73i2.161 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 73, No 2 (2006); 149-152 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/161/156
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