Phylogenetic studies of larval digenean trematodes from freshwater snails and fish species in the proximity of Tshwane metropolitan, South Africa

Onderstepoort Journal of Veterinary Research


 
 
Field Value
 
Title Phylogenetic studies of larval digenean trematodes from freshwater snails and fish species in the proximity of Tshwane metropolitan, South Africa
 
Creator Moema, Esmey B. King, Pieter H. Rakgole, Johnny N.
 
Subject Biology;Parasitology digenean trematodes; classification; description; polymerase chain reaction; PCR; genetic composition; sequence analysis; nucleotide variations; molecular analysis
Description The classification and description of digenean trematodes are commonly accomplished by using morphological features, especially in adult stages. The aim of this study was to provide an analysis of the genetic composition of larval digenean trematodes using polymerase chain reaction (PCR) and sequence analysis. Deoxyribonucleic acid (DNA) was extracted from clinostomatid metacercaria, 27-spined echinostomatid redia, avian schistosome cercaria and strigeid metacercaria from various dams in the proximity of Tshwane metropolitan, South Africa. Polymerase chain reaction was performed using the extracted DNA with primers targeting various regions within the larval digenean trematodes’ genomes. Agarose gel electrophoresis technique was used to visualise the PCR products. The PCR products were sequenced on an Applied Bioinformatics (ABI) genetic analyser platform. Genetic information obtained from this study had a higher degree of discrimination than the morphological characteristics of seemingly similar organisms.
 
Publisher AOSIS
 
Contributor
Date 2019-09-17
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — Experimental
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ojvr.v86i1.1729
 
Source Onderstepoort Journal of Veterinary Research; Vol 86, No 1 (2019); 7 pages 2219-0635 0030-2465
 
Language eng
 
Relation https://ojvr.org/index.php/ojvr/article/view/1729/1986 https://ojvr.org/index.php/ojvr/article/view/1729/1985 https://ojvr.org/index.php/ojvr/article/view/1729/1987 https://ojvr.org/index.php/ojvr/article/view/1729/1983
 
Coverage Freshwater system Phylogenetic Studies 41; Female; Black
Rights Copyright (c) 2019 Esmey B. Moema, Pieter H. King, Johnny N. Rakgole https://creativecommons.org/licenses/by-nd/4.0