Phylogenetic studies of larval digenean trematodes from freshwater snails and fish species in the proximity of Tshwane metropolitan, South Africa
Onderstepoort Journal of Veterinary Research
Field | Value | |
Title | Phylogenetic studies of larval digenean trematodes from freshwater snails and fish species in the proximity of Tshwane metropolitan, South Africa | |
Creator | Moema, Esmey B. King, Pieter H. Rakgole, Johnny N. | |
Description | The classification and description of digenean trematodes are commonly accomplished by using morphological features, especially in adult stages. The aim of this study was to provide an analysis of the genetic composition of larval digenean trematodes using polymerase chain reaction (PCR) and sequence analysis. Deoxyribonucleic acid (DNA) was extracted from clinostomatid metacercaria, 27-spined echinostomatid redia, avian schistosome cercaria and strigeid metacercaria from various dams in the proximity of Tshwane metropolitan, South Africa. Polymerase chain reaction was performed using the extracted DNA with primers targeting various regions within the larval digenean trematodes’ genomes. Agarose gel electrophoresis technique was used to visualise the PCR products. The PCR products were sequenced on an Applied Bioinformatics (ABI) genetic analyser platform. Genetic information obtained from this study had a higher degree of discrimination than the morphological characteristics of seemingly similar organisms. | |
Publisher | AOSIS | |
Date | 2019-09-17 | |
Identifier | 10.4102/ojvr.v86i1.1729 | |
Source | Onderstepoort Journal of Veterinary Research; Vol 86, No 1 (2019); 7 pages 2219-0635 0030-2465 | |
Language | eng | |
Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/1729/1986
https://ojvr.org/index.php/ojvr/article/view/1729/1985
https://ojvr.org/index.php/ojvr/article/view/1729/1987
https://ojvr.org/index.php/ojvr/article/view/1729/1983
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