Use of real-time quantitative reverse transcription polymerase chain reaction for the detection of African horse sickness virus replication in Culicoides imicola
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | Use of real-time quantitative reverse transcription polymerase chain reaction for the detection of African horse sickness virus replication in Culicoides imicola | |
| Creator | Scheffer, Elisabeth G. Venter, Gert J. Joone, Christopher Osterrieder, Nikolaus Guthrie, Alan J. | |
| Description | Despite its important role as vector for African horse sickness virus (AHSV), very little information is available on the dissemination of this virus in Culicoides (Avaritia) imicola Kieffer (Diptera: Ceratopogonidae). This study reports on the applicability of a real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) to detect AHSV in dissected midges. A total of 96 midges were fed on AHSV-infected blood, after which one test group was dissected into head/thorax and abdomen segments immediately after feeding and the other only after 10 days of incubation. The majority of the midges (96%) ingested the virus successfully and there was no significant difference between the virus concentration in the heads/thoraxes and the abdomens immediately after feeding. After incubation, virus was detected in 51% of the midges and it was confined to the abdomen in the majority of these. The fact that virus was detected only in the heads/thoraxes of four Culicoides midges after incubation suggests the presence of a mesenteronal escape barrier. Replication in the salivary glands was not shown. An increase of the mean virus concentration in the abdomen after incubation indicates localised viral replication. The real-time RT-qPCR is recommended for further studies investigating the replication and dissemination of AHSV in Culicoides midges. | |
| Publisher | AOSIS | |
| Date | 2011-11-11 | |
| Identifier | 10.4102/ojvr.v78i1.344 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 78, No 1 (2011); 4 pages 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/344/373
https://ojvr.org/index.php/ojvr/article/view/344/380
https://ojvr.org/index.php/ojvr/article/view/344/374
https://ojvr.org/index.php/ojvr/article/view/344/351
https://ojvr.org/index.php/ojvr/article/downloadSuppFile/344/285
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