Diagnosis and genotyping of African swine fever viruses from 2015 outbreaks in Zambia
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | Diagnosis and genotyping of African swine fever viruses from 2015 outbreaks in Zambia | |
| Creator | Thoromo, Jonas Simulundu, Edgar Chambaro, Herman M. Mataa, Liywalii Lubaba, Caesar H. Pandey, Girja S. Takada, Ayato Misinzo, Gerald Mweene, Aaron S. | |
| Description | In early 2015, a highly fatal haemorrhagic disease of domestic pigs resembling African swine fever (ASF) occurred in North Western, Copperbelt, and Lusaka provinces of Zambia. Molecular diagnosis by polymerase chain reaction targeting specific amplification of p72 (B646L) gene of ASF virus (ASFV) was conducted. Fourteen out of 16 domestic pigs from the affected provinces were found to be positive for ASFV. Phylogenetic analyses based on part of the p72 and the complete p54 (E183L) genes revealed that all the ASFVs detected belonged to genotypes I and Id, respectively. Additionally, epidemiological data suggest that the same ASFV spread from Lusaka to other provinces possibly through uncontrolled and/or illegal pig movements. Although the origin of the ASFV that caused outbreaks in domestic pigs in Zambia could not be ascertained, it appears likely that the virus may have emerged from within the country or region, probably from a sylvatic cycle. It is recommended that surveillance of ASF, strict biosecurity, and quarantine measures be imposed in order to prevent further spread and emergence of new ASF outbreaks in Zambia.Keywords: African swine fever; Asfarviridae; Molecular epidemiology; Zambia | |
| Publisher | AOSIS | |
| Date | 2016-04-29 | |
| Identifier | 10.4102/ojvr.v83i1.1095 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 83, No 1 (2016); 5 pages 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/1095/1412
https://ojvr.org/index.php/ojvr/article/view/1095/1413
https://ojvr.org/index.php/ojvr/article/view/1095/1414
https://ojvr.org/index.php/ojvr/article/view/1095/1406
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