Identification of Echinococcus granulosus strains using polymerase chain reaction–restriction fragment length polymorphism amongst livestock in Moroto district, Uganda

Onderstepoort Journal of Veterinary Research

 
 
Field Value
 
Title Identification of Echinococcus granulosus strains using polymerase chain reaction–restriction fragment length polymorphism amongst livestock in Moroto district, Uganda
 
Creator Chamai, Martin Omadang, Leonard Erume, Joseph Ocaido, Michael Oba, Peter Othieno, Emmanuel Bonaventure, Straton Kitibwa, Annah
 
Subject — —
Description A descriptive study was conducted to identify the different strains of Echinococcus granulosus occurring in livestock in Moroto district, Uganda. Echinococcus cysts from 104 domestic animals, including cattle, sheep, goats and camels, were taken and examined by microscopy, polymerase chain reaction with restriction fragment length polymorphism and Sanger DNA sequencing. Echinococcus granulosus genotypes or strains were identified through use of Bioinformatics tools: BioEdit, BLAST and MEGA6. The major finding of this study was the existence of a limited number of E. granulosus genotypes from cattle, goats, sheep and camels. The most predominant genotype was G1 (96.05%), corresponding to the common sheep strain. To a limited extent (3.95%), the study revealed the existence of Echinococcus canadensis G6/7 in three (n = 3) of the E. granulosus–positive samples. No other strains of E. granulosus were identified. It was concluded that the common sheep strain of Echinococcus sensu stricto and G6/7 of E. canadensis were responsible for echinococcal disease in Moroto district, Uganda.
 
Publisher AOSIS
 
Contributor CESSARi
Date 2016-07-29
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/octet-stream text/xml application/pdf
Identifier 10.4102/ojvr.v83i1.1068
 
Source Onderstepoort Journal of Veterinary Research; Vol 83, No 1 (2016); 7 pages 2219-0635 0030-2465
 
Language eng
 
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The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:

https://ojvr.org/index.php/ojvr/article/view/1068/1510 https://ojvr.org/index.php/ojvr/article/view/1068/1511 https://ojvr.org/index.php/ojvr/article/view/1068/1512 https://ojvr.org/index.php/ojvr/article/view/1068/1499
 
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Rights Copyright (c) 2016 Martin Chamai, Leonard Omadang, Joseph Erume, Michael Ocaido, Peter Oba, Emmanuel Othieno, Straton Bonaventure, Annah Kitibwa https://creativecommons.org/licenses/by-nd/4.0
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