A potential krimpsiekte vaccine
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | A potential krimpsiekte vaccine | |
| Creator | Botha, C.J. Crafford, J.E. Butler Jr., V.P. Stojanovic, M.N. Labuschagne, L. | |
| Description | Krimpsiekte, a chronic form of cardiac glycoside poisoning, is an important plant-induced intoxication of small stock in South Africa. It is caused by cumulative, neurotoxic bufadienolides, such as cotyledoside. A cotyledoside-bovine serum albumin conjugate was synthesized to immunize animals. The efficacy of the cotyledoside-conjugate in inducing an immunological response was ascertained in rabbits (n = 4) and sheep (n = 4) by determining cotyledoside antibody titres with an ELISA using cotyledoside-hen ovalbumin as antigen. The formation of anticotyledoside antibodies was induced in both rabbits and sheep following immunization with the cotyledoside-protein conjugate. Protection provided by the vaccine was demonstrated by challenging sheep (n = 4) with repeated, daily doses of cotyledoside (0.015 mg / kg) administered intravenously, commencing 45 days after the initial vaccination. One control animal died on Day 3 of the challenge period and the other was severely affected after administration of the third cotyledoside dose. The immunized ewes (n = 2) remained clinically unaffected and the challenge was suspended following six daily injections. Vaccination as a means of preventing krimpsiekte seems to be quite feasible and deserves further investigation. | |
| Publisher | AOSIS | |
| Date | 2007-09-13 | |
| Identifier | 10.4102/ojvr.v74i4.117 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 74, No 4 (2007); 307-314 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/117/112
|
|
ADVERTISEMENT
