Immunohaematological reference values for HIV-negative healthy adults in Botswana

African Journal of Laboratory Medicine

Field Value
Title Immunohaematological reference values for HIV-negative healthy adults in Botswana
Creator Mine, Madisa Moyo, Sikhulile Stevens, Penny Michael, Kurt Novitsky, Vladimir Makhaola, Kgomotso Asmelash, Aida Molefhabangwe, S’khatele Woldegabriel, Elias Mothowaeng, Gaseboloke Maruta, Talkmore Kamhukamwe, Charity Mangwendeza, Phibeon M. Holmes-Pretorius, Molly Mtoni, Isaac Motswaledi, Modisa Musonda, Rosemary Ndwapi, Ndwapi Makhema, Joseph Marlink, Richard Seipone, Khumo Gaolathe, Tendani Essex, Max
Subject — Botswana; HIV-negative; Immunohaematology; reference intervals; T-lymphocytes
Description Background: Clinical laboratories in Botswana have relied entirely on the reference intervals for normal immunohaematological values provided by manufacturers’ kits and textbooks. Objectives: The aim of this study was to determine the means, medians, 2.5th and 97.5th percentile reference intervals, for normal immunohaematological values in healthy adults in Botswana.Method: A total of 261 healthy participants comprising 126 men (48%) and 135 (52%) women were enrolled in the southern part of Botswana, and immunological and haematological laboratory parameters were measured.Results: The mean age was 28.8 (95% Confidence Interval [CI] 27.7–29.8) years, with a median of 27 years and a range 18–66 years. The mean haemoglobin level was significantly lower for women (12.4 g/dL; 95% CI 12.1% – 12.7%) than men (15.1 g/dL; 95% CI 14.9% – 15.3%). The women’s haemoglobin reference values (9.0 g/dL – 15.0 g/dL) levels were lower than observed in predominantly White populations (12.0 g/dL – 16.0 g/dL), but comparable with regional consensus reference intervals (9.5 g/dL – 15.8 g/dL) recently defined for East and Southern Africa.Conclusion: The established values provide an important tool for patient management and could influence decisions on inclusion of participants and adverse events in clinical trials conducted locally.
Publisher AOSIS
Date 2011-12-15
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ajlm.v1i1.5
Source African Journal of Laboratory Medicine; Vol 1, No 1 (2012); 7 pages 2225-2010 2225-2002
Language eng
Coverage — — —
Rights Copyright (c) 2011 Madisa Mine, Sikhulile Moyo, Penny Stevens, Kurt Michael, Vladimir Novitsky, Kgomotso Makhaola, Aida Asmelash, S’khatele Molefhabangwe, Elias Woldegabriel, Gaseboloke Mothowaeng, Talkmore Maruta, Charity Kamhukamwe, Phibeon M. Mangwendeza, Molly Holmes-Pretorius, Isaac Mtoni, Modisa Motswaledi, Rosemary Musonda, Ndwapi Ndwapi, Joseph Makhema, Richard Marlink, Khumo Seipone, Tendani Gaolathe, Max Essex