Detection of the bovine viral diarrhoea / mucosal disease (BVD/MD) virus in tissues from aborted ruminant foetuses using immunohistochemistry
Journal of the South African Veterinary Association
| Field | Value | |
| Title | Detection of the bovine viral diarrhoea / mucosal disease (BVD/MD) virus in tissues from aborted ruminant foetuses using immunohistochemistry | |
| Creator | Njiro, S.M. Nkosi, C.M. | |
| Description | Various tissues from aborted ruminant foetuses were collected, fixed in formalin and embedded in paraffin wax. Sections were made and exposed to a primary monoclonal antibody against the bovine viral diarrhoea / mucosal disease (BVD/MD) virus, and subsequently to a goat anti-mouse secondary antibody conjugated to horse radish peroxidase (HRP). Diaminobenzidine (DAB) was the substrate and it released a brown pigment in the tissues on reacting with the HRP in an immunohistochemistry (IHC) procedure. Of 27 aborted foetuses, an immunoperoxidase staining reaction was observed in 1 ovine and 5 bovine foetuses. The IHC procedure located BVD/MD viral antigen in a wide variety of foetal tissues including cerebral cortical neurons, the pseudostratified columnar epithelial cells lining the bronchi, alveolar lining cells and alveolar macrophages, hepatocytes, renal tubular lining cells and the Purkinje fibres in the myocardium. | |
| Publisher | AOSIS | |
| Date | 2009-05-28 | |
| Identifier | 10.4102/jsava.v80i4.213 | |
| Source | Journal of the South African Veterinary Association; Vol 80, No 4 (2009); 229-232 2224-9435 1019-9128 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://jsava.co.za/index.php/jsava/article/view/213/195
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