Microsatellite analysis of cryopreserved stallion semen stored on FTA(R) paper : research communication
Journal of the South African Veterinary Association
| Field | Value | |
| Title | Microsatellite analysis of cryopreserved stallion semen stored on FTA(R) paper : research communication | |
| Creator | Schulman, M.L. Harper, C.K. Bell, E. Nel, A. Guthrie, A.J. | |
| Description | The aim of this study was to establish and validate a method to permit microsatellite analysis of DNA profiles obtained from frozen-thawed stallion sperm cells. This would provide reliable and accurate verification of the identification of a semen donor. Ejaculates from 5 pony stallions were collected, processed and frozen in 0.5 m plastic straws. Aliquots of 100 m of the frozen-thawed semen thus obtained were either placed directly, or diluted (1 : 10 ; 1 : 100 ; and 1 : 1000) and placed on slides of FTA(R) paper. Similarly, blood samples obtained from each of the stallions were placed onto slides of FTA(R) paper. A punch was removed from each sample after drying. Each sample was mixed with FTA(R) purification reagent, Dithiothreitol and Proteinase K before incubation and processing. All samples were processed with a set of 13 microsatellite markers. Further analysis permitted a comparison of the DNA profiles of the frozen-thawed semen and the blood samples. A full profile of markers was obtained from the 1 : 10 and 1 : 100 dilutions of the frozen-thawed semen samples as well as from the blood samples. The DNA profiles from the frozen-thawed semen and blood samples obtained from the stallions matched in all cases. | |
| Publisher | AOSIS | |
| Date | 2002-07-06 | |
| Identifier | 10.4102/jsava.v73i4.592 | |
| Source | Journal of the South African Veterinary Association; Vol 73, No 4 (2002); 222-223 2224-9435 1019-9128 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://jsava.co.za/index.php/jsava/article/view/592/568
|
|
ADVERTISEMENT
