Evaluation of commercial ELISA kits’ diagnostic specificity for FAST diseases in wild animals
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | Evaluation of commercial ELISA kits’ diagnostic specificity for FAST diseases in wild animals | |
| Creator | Milićević, Vesna Glišić, Dimitrije Veljović, Ljubiša Mirčeta, Jovan Kureljušić, Branislav Đorđević, Milutin Vasković, Nikola | |
| Description | Wild animals, sharing pathogens with domestic animals, play a crucial role in the epidemiology of infectious diseases. Sampling from wild animals poses significant challenges, yet it is vital for inclusion in disease surveillance and monitoring programmes. Often, mass surveillance involves serological screenings using enzyme-linked immunosorbent assay (ELISA) tests, typically validated only for domestic animals. This study assessed the diagnostic specificity of commercially available ELISA tests on 342 wild ruminant serum samples and 100 from wild boars. We evaluated three tests for foot-and-mouth disease: two for Peste des petits ruminants, two for Rift Valley fever and one for Capripox virus. Diagnostic specificity was calculated using the formula True Negative/(False Positive + True Negative). Cohen’s kappa coefficient measured agreement between tests. Results showed high specificity and agreement across all tests. Specificity for foot-and-mouth disease (FMD) ranged from 93.89% for Prionics to 100% for IDEXX, with IDvet showing 99.6%. The highest agreement was between FMD IDvet and IDEXX at 97.1%. Rift Valley fever (RVF) tests, Ingezim and IDvet, achieved specificities of 100% and 98.83%, respectively. The optimal specificity was attained by retesting single reactors and inactivating the complement.Contribution: Commercially available ELISA kits are specific for foot-and-mouth disease and similar transboundary animal diseases and can be used for highly specific wild animal testing. | |
| Publisher | AOSIS | |
| Date | 2024-06-28 | |
| Identifier | 10.4102/ojvr.v91i1.2164 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 91, No 1 (2024); 7 pages 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/2164/2593
https://ojvr.org/index.php/ojvr/article/view/2164/2594
https://ojvr.org/index.php/ojvr/article/view/2164/2595
https://ojvr.org/index.php/ojvr/article/view/2164/2596
|
|
ADVERTISEMENT
