Hepatoprotective effects of leaf extract of Annona senegalensis against aflatoxin B1 toxicity in rats

Onderstepoort Journal of Veterinary Research

Field Value
Title Hepatoprotective effects of leaf extract of Annona senegalensis against aflatoxin B1 toxicity in rats
Creator Makhuvele, Rhulani Foubert, Kenn Hermans, Nina Pieters, Luc Verschaeve, Luc Elgorashi, Esameldin
Subject Science; Toxicology; Phytomedicine; Animal Health aflatoxin B1; toxicity; hepatoprotective activity; Sprague-Dawley rats, Annona senegalensis
Description Global aflatoxin contamination of agricultural commodities is of the most concern in food safety and quality. This study investigated the hepatoprotective effect of 80% methanolic leaf extract of Annona senegalensis against aflatoxin B1 (AFB1)-induced toxicity in rats. A. senegalensis has shown to inhibit genotoxicity of aflatoxin B1 in vitro. The rats were divided into six groups including untreated control, aflatoxin B1 only (negative control); curcumin (positive control; 10 mg/kg); and three groups receiving different doses (100 mg/kg, 200 mg/kg, and 300 mg/kg) of A. senegalensis extract. The rats received treatment (with the exception of untreated group) for 7 days prior to intoxication with aflatoxin B1. Serum levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, and creatinine were measured. Hepatic tissues were analysed for histological alterations. Administration of A. senegalensis extract demonstrated hepatoprotective effects against aflatoxin B1-induced toxicity in vivo by significantly reducing the level of serum aspartate aminotransferase and alanine aminotransferase and regenerating the hepatocytes. No significant changes were observed in the levels of alkaline phosphatase, lactate dehydrogenase, and creatinine for the AFB1 intoxicated group, curcumin+AFB1 and Annona senegalensis leaf extract (ASLE)+AFB1 (100 mg/kg, 200 mg/kg, and 300 mg/kg body weight [b.w.]) treated groups. Annona senegalensis is a good candidate for hepatoprotective agents and thus its use in traditional medicine may at least in part be justified.Contribution: The plant extract investigated in this study can be used in animal health to protect the organism from toxicity caused by mycotoxins.
Publisher AOSIS
Contributor National Research Foundation (NRF Fonds Wetenschappelijk Onderzoek (FWO) Flemish Interuniversity Council (VLIR) Agricultural Research Council (ARC-OVR)
Date 2024-03-11
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — Experimental
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ojvr.v91i1.2133
Source Onderstepoort Journal of Veterinary Research; Vol 91, No 1 (2024); 6 pages 2219-0635 0030-2465
Language eng
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https://ojvr.org/index.php/ojvr/article/view/2133/2568 https://ojvr.org/index.php/ojvr/article/view/2133/2569 https://ojvr.org/index.php/ojvr/article/view/2133/2570 https://ojvr.org/index.php/ojvr/article/view/2133/2571
Coverage — — Age; Gender; Ethnicity
Rights Copyright (c) 2024 Rhulani Makhuvele, Kenn Foubert, Nina Hermans, Luc Pieters, Luc Verschaeve, Esameldin Elgorashi https://creativecommons.org/licenses/by/4.0