Role of CTX-M-15 gene in spread of extended-spectrum beta-lactamases among immunocompetent patients in Ghana

African Journal of Laboratory Medicine

 
 
Field Value
 
Title Role of CTX-M-15 gene in spread of extended-spectrum beta-lactamases among immunocompetent patients in Ghana
 
Creator Obeng-Nkrumah, Noah Tawiah-Abrokwa, Gloria D. Owusu, Enid Duah, Francisca Oduro-Mensah, Daniel Kwao, Paul Evariste, Bako Labi, Appiah-Korang
 
Subject Microbiology; Bacteriology; Antibiotic drug resistance extended-spectrum beta-lactamases; prevalence; inpatient; faecal carriage; Enterobacterales; Ghana
Description Background: Patients with faecal carriage of extended-spectrum beta-lactamases (ESBL)-producing Enterobacterales serve as reservoirs and sources of dissemination and infection.Objective: This report examined immunocompetent patients for faecal carriage of ESBL-producing Enterobacterales in a district care hospital setting in Ghana.Methods: Between March 2019 and May 2020, cross-sectional sampling was performed to enrol patients and conduct questionnaire-structured interviews for factors that predispose patients to ESBL faecal carriage. Faecal samples from study patients were quantified for ESBL-producing Enterobacterales. The ESBL genes were characterised by polymerase chain reaction and sequencing.Results: The overall proportion of ESBL faecal carriage was 35.5% (n = 38/107). The blaCTX-M gene, mostly CTX-M-15, was detected in 89.5% (n = 34/38) of the ESBL-producing isolates. The other ESBL types included blaSHV (n = 3) and blaOXA (n = 1). The CTX-M-15-positive isolates, when present in a faecal sample compared to the non-ESBL-CTX-M-15 isolates, constituted the predominant faecal Enterobacterales, with significantly higher colony counts than all other enterobacteria in that sample. In multivariate regression, independent risk factors for faecal carriage of ESBL-producing Enterobacterales were hospitalisation in the past year, infections since admission, use of antibiotics in the past 6 weeks, and admission from another hospital.Conclusion: The study found that CTX-M-15-producing isolates were the predominant faecal Enterobacterales, and that further investigations are needed to determine the reasons behind this dominance.What this study adds: The CTX-M-15-producing isolates dominance in this study shows the misuse and abuse of antibiotics in an African medical facility and indicates the potential role of immunity in controlling ESBL spread, which is to be investigated further.
 
Publisher AOSIS
 
Contributor University of Ghana, Carnegie Building a New Generation of Academics in Africa Project, The Carnegie Corporation of New York.
Date 2023-11-20
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — Cross-sectional; questionnaire interviews
Format text/html application/epub+zip text/xml application/pdf application/pdf
Identifier 10.4102/ajlm.v12i1.2135
 
Source African Journal of Laboratory Medicine; Vol 12, No 1 (2023); 10 pages 2225-2010 2225-2002
 
Language eng
 
Relation
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https://ajlmonline.org/index.php/ajlm/article/view/2135/2780 https://ajlmonline.org/index.php/ajlm/article/view/2135/2781 https://ajlmonline.org/index.php/ajlm/article/view/2135/2782 https://ajlmonline.org/index.php/ajlm/article/view/2135/2783 https://ajlmonline.org/index.php/ajlm/article/view/2135/2831
 
Coverage Africa Ghana Inpatients; faecal specimens; questionnaire interviews
Rights Copyright (c) 2023 Noah Obeng-Nkrumah, Gloria D. Tawiah-Abrokwa, Enid Owusu, Francisca Duah, Daniel Oduro-Mensah, Paul Kwao, Bako Evariste, Appiah-Korang Labi https://creativecommons.org/licenses/by/4.0
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