Monitoring the genetic variation of some Escherichia coli strains in wild birds and cattle

Onderstepoort Journal of Veterinary Research

Field Value
Title Monitoring the genetic variation of some Escherichia coli strains in wild birds and cattle
Creator Ibrahim, Ghada A. Salah-Eldein, Ahmed M. Al-zaban, Mayasar I. El-Oksh, Amal S.A. Ahmed, Elsayyad M. Farid, Doaa S. Saad, Enas M.
Subject Wildlife; Bacteriology; Environmental science E. coli; wild birds; cattle; virulence genes; resistant genes; PCR; antibiotics; sequencing
Description To date, there is limited data about the genetic relationship of Escherichia coli between wild birds and cattle because these birds act as silent vectors for many zoonotic bacteria. This study aimed to elucidate the role of rooming wild birds in the vicinity of cattle farm in transmission of the same pathogenic E. coli variants, identifying their virulence, resistance traits and genetic similarities of fimH virulence gene. About 240 faecal/cloacal swabs were collected from both species and examined bacteriologically. Escherichia coli was yielded in 45.8% and 32.5%, respectively, of examined cattle and wild birds. The most prevalent detected E. coli serovar was O26. High tetracycline and chloramphenicol resistance were recorded; however, gentamycin and ciprofloxacin exhibited the highest sensitivity rates. Polymerase chain reaction (PCR) conserved genotypic resistance (tetA and blaCTX-M) and virulence attributes (fimH, stx1, eaeA and ompA) of E. coli isolates were discussed in detail. The fimH gene revealed 100% sequence similarity when comparing with different E. coli isolates globally and locally. Finally, a close genetic association of E. coli with both wild birds and cattle was detected, thus strengthening its role in the dissemination of the infection via environment. Prevention and conservative policy should be carried as E. coli constitute enormous significant zoonotic risks to livestock and animal workers. Also, further studies to the whole genome sequencing of fimH, other virulence and resistance genes of E. coli are recommended trying to limit the possibilities of co-infection and transfer among different species.Contribution: The current study recorded updated data about the critical infectious role of wild birds to livestock, including cattle farms in Egypt. It also delivered some recommendations for good hygienic practices in cattle farms which must be implemented for handling animal manure.
Publisher AOSIS
Contributor personal funding
Date 2023-07-26
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ojvr.v90i1.2085
Source Onderstepoort Journal of Veterinary Research; Vol 90, No 1 (2023); 10 pages 2219-0635 0030-2465
Language eng
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
Coverage — — —
Rights Copyright (c) 2023 Ghada A. Ibrahim, Ahmed M. Salah-Eldein, Mayasar I. Al-zaban, Amal S.A. El-Oksh, Elsayyad M. Ahmed, Doaa S. Farid, Enas M. Saad