Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania

African Journal of Laboratory Medicine

 
 
Field Value
 
Title Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania
 
Creator Mwakyabala, Jenipher G. Mtemisika, Conjester I. Mshana, Stacy Mwakyoma, Adam A. Silago, Vitus
 
Subject — beta lactamases; Gram-negative bacteria; healthcare associated infections; multiplex PCR assay
Description Healthcare-associated infections (HCAIs) caused by extended spectrum β-lactamase-producing Gram-negative bacteria (ESBL-GNB) increase morbidity and mortality. This cross-sectional study characterised ESBL genes (blaCTX-M, blaTEM and blaSHV) among 30 ceftriaxone-resistant GNB causing HCAIs between January 2022 and July 2022 by multiplex polymerase chain reaction assay at the zonal referral hospital in Mwanza, Tanzania. Twenty-five (83.3%) had at least one ESBL gene, of which 23/25 (92.0%) carried the blaCTX-M gene. Seventy-two percent (18/25) of the GNB-ESBL isolates carried more than one ESBL gene, of which the majority (88.8%; n = 16/25) carried the blaCTX-M and blaTEM genes. Extended spectrum β-lactamase genes, particularly blaCTX-M, are common among ceftriaxone-resistant GNB causing HCAIs.What this study adds: This study revealed the distribution of genes (blaCTX-M, blaTEM and blaSHV) coding for ESBL production among ceftriaxone resistant GNB causing HCAIs However, all ESBL producing GNB were susceptible towards ceftriaxone-sulbactam indicating that ceftriaxone-sulbactam may be empirically prescribed for treating patients with HCAIs.
 
Publisher AOSIS
 
Contributor —
Date 2023-04-12
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ajlm.v12i1.2107
 
Source African Journal of Laboratory Medicine; Vol 12, No 1 (2023); 5 pages 2225-2010 2225-2002
 
Language eng
 
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https://ajlmonline.org/index.php/ajlm/article/view/2107/2616 https://ajlmonline.org/index.php/ajlm/article/view/2107/2617 https://ajlmonline.org/index.php/ajlm/article/view/2107/2618 https://ajlmonline.org/index.php/ajlm/article/view/2107/2619
 
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Rights Copyright (c) 2023 Jenipher https://creativecommons.org/licenses/by/4.0
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