Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka

African Journal of Laboratory Medicine

 
 
Field Value
 
Title Alternative methods for calculating percentage haemolysis of red cell concentrates in peripheral blood banks in Sri Lanka
 
Creator Fernando, Caroline A. Dissanayake, Deklanji T. Hewamana, Uththara I. Rathnaweera, Shyamini Samanthilake, Wickrama A. Tudugala, Ranga Jayasekara, Kithsiri B. Kuruppu, Kumudu
 
Subject Allied Health Sciences; Medical Laboratory Science blood banks; capillary tube comparison; haemoglobin colour scale; percentage haemolysis; red cell concentrate
Description Background: Haemolysis – one of the major limiting factors of red cell concentrate quality – must be measured as a quality-monitoring requirement. According to international quality standards, percentage haemolysis must be monitored in 1.0% of red cell concentrates produced monthly and maintained under 0.8%.Objective: This study assessed three alternative methods for determining plasma haemoglobin concentration in peripheral blood banks that lack a plasma or low haemoglobin photometer – the gold-standard method – in Sri Lanka.Methods: A standard haemolysate was prepared using an unexpired whole blood pack of normal haemoglobin concentration. A concentration series from 0.1 g/dL to 1.0 g/dL was prepared by diluting portions of standard haemolysate with saline. The alternative methods, namely visual haemoglobin colour scale, spectrophotometric calibration graph, and standard haemolysate capillary tube comparison, were designed using this concentration series and were used to test red cell concentrates received at the Quality Control Department of the National Blood Center, Sri Lanka, from February 2021 to May 2021.Results: A strong correlation was observed between the haemoglobin photometer method and the alternative methods (R = ~0.9). Based on the linear regression model, the standard haemolysate capillary tube comparison method was the best of the three alternative methods (R2 = 0.974).Conclusion: All three alternative methods are recommended for use in peripheral blood banks. The standard haemolysate capillary tube comparison method was the best model. 
 
Publisher AOSIS
 
Contributor
Date 2023-02-23
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ajlm.v12i1.1987
 
Source African Journal of Laboratory Medicine; Vol 12, No 1 (2023); 7 pages 2225-2010 2225-2002
 
Language eng
 
Relation
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:

https://ajlmonline.org/index.php/ajlm/article/view/1987/2578 https://ajlmonline.org/index.php/ajlm/article/view/1987/2579 https://ajlmonline.org/index.php/ajlm/article/view/1987/2580 https://ajlmonline.org/index.php/ajlm/article/view/1987/2581
 
Coverage Sri Lanka; Western Province; — Age; Gender
Rights Copyright (c) 2023 Caroline A. Fernando, Deklanji T. Dissanayake, Uththara I. Hewamana, Shyamini Rathnaweera, Wickrama A. Samanthilake, Ranga Tudugala, Kithsiri B. Jayasekara, Kumudu Kuruppu https://creativecommons.org/licenses/by/4.0
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