Effect of sputum quality and role of Xpert® MTB/RIF assay for detection of smear-negative pulmonary tuberculosis in same-day diagnosis strategy in Addis Ababa, Ethiopia

African Journal of Laboratory Medicine

 
 
Field Value
 
Title Effect of sputum quality and role of Xpert® MTB/RIF assay for detection of smear-negative pulmonary tuberculosis in same-day diagnosis strategy in Addis Ababa, Ethiopia
 
Creator Sinshaw, Waganeh Kebede, Abebaw Bitew, Adane Tadesse, Mengistu Mehamed, Zemedu Alemu, Ayinalem Yenew, Bazezew Amare, Misikir Dagne, Biniyam Diriba, Getu Tesfaye, Ephrem Gamtesa, Dinka F. Abebaw, Yeshiwork Mollalign, Helina Seid, Getachew Getahun, Muluwork
 
Subject — smear-negative pulmonary tuberculosis; Xpert® MTB/RIF test; sputum quality; tuberculosis culture; diagnostic performance
Description Background: There is limited information on the performance of the Xpert® MTB/RIF test for diagnosis of smear-negative pulmonary tuberculosis (SNPT) and rifampicin resistance (RR) in the same-day diagnosis approach. The effects of sputum quality and other factors affecting the Xpert performance are also under-investigated.Objective: This study aimed to determine the performance of the Xpert® MTB/RIF test for detection of SNPT and RR in the same-day diagnosis strategy and the effect of sputum quality and other factors on its performance.Methods: A cross-sectional study was conducted from August 2017 to January 2018 across 16 health facilities in Addis Ababa, Ethiopia. Two spot sputum samples were collected from 418 presumptive SNPT patients, tested with Xpert® MTB/RIF, then compared to tuberculosis culture. Additionally, culture isolates were tested for RR by BACTEC MGIT™ 960 drug susceptibility testing (DST) and MTBDRplus version 2.Results: The Xpert® MTB/RIF test detected 24 (5.7%) SNPT cases, with a sensitivity of 92.3% (75.9% – 97.9%) and specificity of 99.2% (97.8% – 99.7%) compared with tuberculosis culture. Xpert® MTB/RIF also detected three (11.58%) RR strains with 100.0% concordance with BACTEC MGIT™ 960 DST and MTBDRplus results. Three blood-stained SNPT samples were positive by Xpert (30.0%), which was 6.9 times higher compared to salivary sputum (odds ratio: 6.9, 95% confidence interval: 1.36–34.96, p = 0.020).Conclusion: The performance of the Xpert® MTB/RIF to detect SNPT and RR in same-day diagnosis is high. However, SNPT positivity varies among sputum qualities, and good sample collection is necessary for better test performance.
 
Publisher AOSIS
 
Contributor
Date 2022-08-31
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf application/pdf
Identifier 10.4102/ajlm.v11i1.1671
 
Source African Journal of Laboratory Medicine; Vol 11, No 1 (2022); 7 pages 2225-2010 2225-2002
 
Language eng
 
Relation
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:

https://ajlmonline.org/index.php/ajlm/article/view/1671/2381 https://ajlmonline.org/index.php/ajlm/article/view/1671/2382 https://ajlmonline.org/index.php/ajlm/article/view/1671/2383 https://ajlmonline.org/index.php/ajlm/article/view/1671/2385 https://ajlmonline.org/index.php/ajlm/article/view/1671/2384
 
Coverage — — —
Rights Copyright (c) 2022 Waganeh Sinshaw, Abebaw Kebede, Adane Bitew, Mengistu Tadesse, Zemedu Mehamed, Ayinalem Alemu, Bazezew Yenew, Misikir Amare, Biniyam Dagne, Getu Diriba, Ephrem Tesfaye, Dinka F. Gamtesa, Yeshiwork Abebaw, Helina Molallign Mollalign, Getachew Seid, Muluwor https://creativecommons.org/licenses/by/4.0
ADVERTISEMENT