Species identification and cow risks of non-aureus staphylococci from South African dairy herds

Onderstepoort Journal of Veterinary Research

 
 
Field Value
 
Title Species identification and cow risks of non-aureus staphylococci from South African dairy herds
 
Creator Petzer, Inge-Marie Labuschagne, Christiaan Phophi, Lufuno Karzis, Joanne
 
Subject Veterinary science; microbiology; mastitis molecular epidemiology; NAS species identification; genetic diversity; cow risk factors; intramammary infection; dairy cows; South Africa
Description Detailed information on specific species of non-aureus staphylococci (NAS) has become a necessity for effective udder health control programs in South Africa. The main objective of this preliminary study was to identify the different NAS species and strains present in dairy herds in South Africa using a cost-effective method. A further objective was to investigate the effects of cow risk factors and farming systems on the NAS isolates identified. A total of 214 NAS, isolated from milk collected from 17 South African dairy herds, were identified using three diagnostic tests (API Staph test, MALDI-TOF and 16s rRNA). There was a good observed agreement between the MALDI-TOF and 16S rRNA sequencing (92.2%) and a poor observed agreement between the MALDI-TOF and API Staph (25.7%). The genetic relatedness within species was investigated in 128 of these isolates using random polymorphic amplified deoxyribonucleic acid (DNA) (RAPD), verified by multilocus sequence typing (MLST), and phylogenetic analysis and cow risk factors were investigated on species level. The main NAS species isolated were Staphylococcus chromogenes (75.2%), Staphylococcus epidermidis (9.4%) and Staphylococcus haemolyticus (8.9%). The RAPD test identified 34 Staphylococcus chromogenes, 13 Staphylococcus epidermidis and nine Staphylococcus haemolyticus strains, indicating genetic diversity amongst strains and herds. The presence of NAS intramammary infections was found to be significantly related to the farming systems, composite cow milk somatic cell count (SCC), parity and days in milk (DIM). Significantly more NAS were isolated from primiparous and from older cows. This knowledge could assist with the management of NAS on dairy farms.
 
Publisher AOSIS
 
Contributor This research was partially funded by The National Research Foundation of South Africa (Grant number: 120319) and Milk SA (Grant number: PRJ 0212). The funding bodies had no part in the study design, collection, analysis and data interpretation and the ma
Date 2022-07-27
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — Research article
Format text/html application/epub+zip text/xml application/pdf application/pdf
Identifier 10.4102/ojvr.v89i1.2021
 
Source Onderstepoort Journal of Veterinary Research; Vol 89, No 1 (2022); 10 pages 2219-0635 0030-2465
 
Language eng
 
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The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:

https://ojvr.org/index.php/ojvr/article/view/2021/2366 https://ojvr.org/index.php/ojvr/article/view/2021/2367 https://ojvr.org/index.php/ojvr/article/view/2021/2368 https://ojvr.org/index.php/ojvr/article/view/2021/2370 https://ojvr.org/index.php/ojvr/article/view/2021/2369
 
Coverage South Africa present day Bovine milk samples, bacteria isolates
Rights Copyright (c) 2022 Inge-Marie Petzer, Christiaan Labuschagne, Lufuno Phophi, Joanne Karzis https://creativecommons.org/licenses/by/4.0
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