Gibberellic acid influences growth indices and biochemical parameters in micropropagated Ocimum gratissimum L. explants

Journal of Medicinal Plants for Economic Development

 
 
Field Value
 
Title Gibberellic acid influences growth indices and biochemical parameters in micropropagated Ocimum gratissimum L. explants
 
Creator Olaitan, Muyibat M. Mangse, George Ogbaga, Chukwuma C. Uthman, Taofik O.
 
Subject — plant tissue culture; plant growth regulators; explant; micropropagation; antioxidants; somatic embryogenesis
Description Background: Ocimum gratissimum L. is a well-known tropical and subtropical plant widely utilised for both medicinal and nutritional purposes. However, its continuous existence is currently threatened because of excessive human exploitation and non-regulated collection.Aim: This study aimed at mass-producing O. gratissimum through its explants cultured on Murashige and Skoog (MS) medium.Settings: Sterile explants of O. gratissimum were used to generate whole plant through the process of somatic embryogenesis and under the influence of different plant growth regulators (PGRs).Methods: The growth medium was supplemented with various concentrations (0.1 mg/L, 0.5 mg/L and 1.0 mg/L) of PGRs, namely, gibberellic acid (GA3) and benzyl aminopurine (BAP) in combination with 0.2 mg/L indole acetic acid (IAA), which also served as the control.Results: The results showed that germination response, shoot and root lengths were significantly enhanced in O. gratissimum explants raised in media containing the two PGRs in a concentration-dependent manner after four weeks of culture. Ocimum gratissimum explants treated with GA3 and BAP also experienced reduced lipid peroxidation and ascorbic acid concentration, particularly at the highest tested concentration (1.0 mg/L) as evidenced by the significant drop in malondialdehyde (MDA) concentration. In response to this, the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly increased in O. gratissimum explants raised in MS medium supplemented with PGRs.Conclusion: These results generally suggest that GA3 in combination with IAA is more favourable than BAP for the micropropagation of O. gratissimum explants. Thus, our study revealed that PGRs possess special attributes, which could be exploited in tissue culture for the micropropagation of O. gratissimum explants.
 
Publisher AOSIS Publishing
 
Contributor
Date 2022-07-26
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — Qualitative research
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/jomped.v6i1.154
 
Source Journal of Medicinal Plants for Economic Development; Vol 6, No 1 (2022); 7 pages 2616-4809 2519-559X
 
Language eng
 
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https://jomped.org/index.php/jomped/article/view/154/482 https://jomped.org/index.php/jomped/article/view/154/483 https://jomped.org/index.php/jomped/article/view/154/484 https://jomped.org/index.php/jomped/article/view/154/485
 
Coverage Africa — Plants
Rights Copyright (c) 2022 Muyibat M. Olaitan, George Mangse, Chukwuma C. Ogbaga, Taofik O. Uthman https://creativecommons.org/licenses/by/4.0
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