Creation of a contusion injury in rabbit skeletal muscle using a drop-mass technique
Journal of the South African Veterinary Association
| Field | Value | |
| Title | Creation of a contusion injury in rabbit skeletal muscle using a drop-mass technique | |
| Creator | Deane, Margaret N. Gregory, Michael Mars, Maurice Bester, Linda | |
| Description | This study reports our experience in developing a simple, minor injury. After reviewing the literature, a ‘drop-mass’ method was selected where a 201 g, elongated oval-shaped weight was dropped up to 15 times through a 1 m tube onto the left vastus lateralis of New Zealand white rabbits. To determine the extent of injury and degree of healing, biopsies were obtained six days after injury from the healing vastus lateralis of each animal. The tissue was fixed in formal saline, embedded in wax, cut and stained with haematoxylin and eosin (HE) and phosphotungstic acid haematoxylin (PTAH) and examined by light microscopy (LM). The ‘optimal’ injury was created after seven drops, where quite severe, mild and moderately severe trauma was caused to muscle in the juxta-bone, mid and sub-dermal regions respectively. In each region, the muscle exhibited features of healing six days after injury. The ‘drop-mass’ technique appears to cause a contusion within a single muscle of at least three degrees of severity. This previously unreported observation is of particular importance to other researchers wishing to investigate contusion injury in other animal models. | |
| Publisher | AOSIS | |
| Date | 2013-08-13 | |
| Identifier | 10.4102/jsava.v84i1.957 | |
| Source | Journal of the South African Veterinary Association; Vol 84, No 1 (2013); 6 pages 2224-9435 1019-9128 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://jsava.co.za/index.php/jsava/article/view/957/1217
https://jsava.co.za/index.php/jsava/article/view/957/1218
https://jsava.co.za/index.php/jsava/article/view/957/1219
https://jsava.co.za/index.php/jsava/article/view/957/1216
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