Effects of TNF-α and IL-10-819 T>C single nucleotide polymorphisms on urogenital schistosomiasis in preschool children in Zimbabwe

African Journal of Laboratory Medicine

Field Value
Title Effects of TNF-α and IL-10-819 T>C single nucleotide polymorphisms on urogenital schistosomiasis in preschool children in Zimbabwe
Creator Marume, Amos Chimponda, Theresa Vengesai, Arthur Mushayi, Caroline Mann, Jaclyn Mduluza, Takafira
Subject — Schistosoma haematobium; polymorphisms; cytokines; susceptibility; protective immunity
Description Background: Knowledge gaps exist between host genetic factors and susceptibility to schistosomiasis.Objective: This study determined cytokine levels and single nucleotide polymorphisms of tumour necrosis factor (TNF)-α (rs1800629) and interleukin (IL)-10 (rs1800871) and their possible impact on susceptibility to schistosomiasis in preschool-age children in the Madziva area of Shamva district, Mashonaland Central province, Zimbabwe.Methods: Urogenital schistosomiasis was diagnosed using the urine filtration method, while a sandwich enzyme-linked immunosorbent assay was used for cytokine level determination. The survey was done in August 2015 and reinfection levels post treatment were assessed at 3, 6 and 12 months. Amplification refractory mutation system polymerase chain reaction with visualisation on 2% agarose gel electrophoresis was used for genotyping.Results: Schistosomiasis prevalence was found to be 10.5% (59/563). Reinfections were detected in only six children at 3 months and only one was reinfected at 12 months. There were no significant differences in TNF-α-308 G/A allele or genotype frequencies between the Schistosoma haematobium infected participants (p = 0.360) and uninfected participants (p = 0.279). However, no children with the IL-10-819 TT genotype had schistosomiasis. The TNF-α GG genotype corresponded with significantly lower TNF-α levels when compared with the GA or AA genotypes (p 0.001), and TNF-α levels were significantly lower in infected children compared to uninfected children (p 0.001).Conclusion: Higher TNF-α levels and lower IL-10 levels are potentially protective against schistosomiasis infection. The IL-10-819 TT genotype is potentially protective against infection through its association with lower IL-10 levels.
Publisher AOSIS
Date 2021-04-29
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ajlm.v10i1.1138
Source African Journal of Laboratory Medicine; Vol 10, No 1 (2021); 7 pages 2225-2010 2225-2002
Language eng
Relation https://ajlmonline.org/index.php/ajlm/article/view/1138/1952 https://ajlmonline.org/index.php/ajlm/article/view/1138/1951 https://ajlmonline.org/index.php/ajlm/article/view/1138/1953 https://ajlmonline.org/index.php/ajlm/article/view/1138/1950
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Rights Copyright (c) 2021 Amos Marume, Theresa Chimponda, Arthur Vengesai, Caroline Mushayi, Jaclyn Mann, Takafira Mduluza https://creativecommons.org/licenses/by/4.0