Performance of TaqMan probes for the detection of sexually transmitted infections in South African women

African Journal of Laboratory Medicine


 
 
Field Value
 
Title Performance of TaqMan probes for the detection of sexually transmitted infections in South African women
 
Creator Mitchev, Nireshni Singh, Ravesh Garrett, Nigel Ramsuran, Veron Niehaus, Abraham J. Mlisana, Koleka P.
 
Subject Medicine; Microbiology; Molecular sexually transmitted infections; vaginal discharge syndrome; molecular diagnostics; validation; TaqMan.
Description Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis and Mycoplasma genitalium are the four main aetiologies of sexually transmitted infections responsible for vaginal discharge syndrome (VDS). Commercially available multiplex polymerase chain reaction (PCR) assays are expensive and generally not customisable. We evaluated a highly customisable singleplex PCR approach by testing it in parallel with the Anyplex™ II STI-7 detection assay in a cohort of South African women that presented with VDS between May 2016 and January 2017. Our multiple singleplex PCR strategy proved to be a simple, accurate, rapid, affordable and scalable option for diagnosing VDS.
 
Publisher AOSIS
 
Contributor
Date 2021-03-31
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ajlm.v10i1.1124
 
Source African Journal of Laboratory Medicine; Vol 10, No 1 (2021); 4 pages 2225-2010 2225-2002
 
Language eng
 
Relation https://ajlmonline.org/index.php/ajlm/article/view/1124/1934 https://ajlmonline.org/index.php/ajlm/article/view/1124/1933 https://ajlmonline.org/index.php/ajlm/article/view/1124/1935 https://ajlmonline.org/index.php/ajlm/article/view/1124/1932
 
Coverage Durban — —
Rights Copyright (c) 2021 Nireshni Mitchev, Ravesh Singh, Nigel Garrett, Veron Ramsuran, Abraham J. Niehaus, Koleka P. Mlisana https://creativecommons.org/licenses/by/4.0