Analysis of antibiotic resistance phenotypes and genes of Escherichia coli from healthy swine in Guizhou, China

Onderstepoort Journal of Veterinary Research


 
 
Field Value
 
Title Analysis of antibiotic resistance phenotypes and genes of Escherichia coli from healthy swine in Guizhou, China
 
Creator Yu, Bo Zhang, Yanan Yang, Li Xu, Jinge Bu, Shijin
 
Subject — antimicrobial resistance; extended-spectrum β-lactamase; ESBLs; E. coli from swine; plasmid-mediated quinolone resistance; PMQR; resistance genes
Description This study was carried out to investigate the resistance phenotypes and resistance genes of Escherichia coli from swine in Guizhou, China. A total of 47 E. coli strains isolated between 2013 and 2018 were tested using the Kirby–Bauer (K–B) method to verify their resistance to 19 common clinical antimicrobials. Five classes consisting of 29 resistance genes were detected using polymerase chain reaction. The status regarding extended-spectrum β-lactamase (ESBL) and the relationship between ESBL CTX-M-type β-lactamase genes and plasmid-mediated quinolone resistance (PMQR) genes were analysed. A total of 46 strains (97.9%) were found to be multidrug resistant. Amongst them, 27 strains (57.4%) were resistant to more than eight antimicrobials, and the maximum number of resistant antimicrobial agents was 16. Twenty antibiotic resistance genes were detected, including six β-lactamase genes blaTEM (74.5%), blaCTX-M-9G (29.8%), blaDHA (17.0%), blaCTX-M-1G (10.6%), blaSHV (8.5%), blaOXA (2.1%), five aminoglycoside-modifying enzyme genes aac(3′)-IV (93.6%), aadA1 (78.7%), aadA2 (76.6%), aac(3′)-II c (55.3%), aac(6′)-Ib (2.1%) and five amphenicol resistance genes floR (70.2%), cmlA (53.2%), cat2 (10.6%), cat1 (6.4%), cmlB (2.1%), three PMQR genes qnrS (55.3%), oqxA (53.2%), qepA (27.7%) and polypeptide resistance gene mcr-1 (40.4%). The detection rate of ESBL-positive strains was 80.9% (38/47) and ESBL TEM-type was the most abundant ESBLs. The percentage of the PMQR gene in blaCTX-M-positive strains was high, and the detection rate of blaCTX-M-9G was the highest in CTX-M type. It is clear that multiple drug resistant E. coli is common in healthy swine in this study. Extended-spectrum β-lactamase is very abundant in the E. coli strains isolated from swine and most of them are multiple compound genotypes.
 
Publisher AOSIS
 
Contributor
Date 2021-02-24
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ojvr.v88i1.1880
 
Source Onderstepoort Journal of Veterinary Research; Vol 88, No 1 (2021); 8 pages 2219-0635 0030-2465
 
Language eng
 
Relation https://ojvr.org/index.php/ojvr/article/view/1880/2212 https://ojvr.org/index.php/ojvr/article/view/1880/2211 https://ojvr.org/index.php/ojvr/article/view/1880/2213 https://ojvr.org/index.php/ojvr/article/view/1880/2210
 
Coverage — — —
Rights Copyright (c) 2021 Shijin Bu https://creativecommons.org/licenses/by-nd/4.0