Development of dried tube specimens for Xpert MTB/RIF proficiency testing
African Journal of Laboratory Medicine
| Field | Value | |
| Title | Development of dried tube specimens for Xpert MTB/RIF proficiency testing | |
| Creator | DeGruy, Kyle Klein, Katherine Rey, Zilma Hall, Patricia Kim, Andrea Alexander, Heather | |
| Description | Background: Proficiency testing (PT) is part of a comprehensive quality assurance programme, which is critical to ensuring patients receive accurate and reliable diagnostic testing. Implementation of the Cepheid Xpert® MTB/RIF assay to aid in the diagnosis of tuberculosis has expanded rapidly in recent years; however, PT material for Xpert MTB/RIF is not readily available in many resource-limited settings.Objective: To develop an accurate and precise PT material based on the dried tube specimen (DTS) method, using supplies and reagents available in most tuberculosis culture laboratories.Methods: Dried tube specimens were produced at the United States Centers for Disease Control and Prevention from 2013 to 2015 by inactivating liquid cultures of well-characterised mycobacterial strains. Ten percent of DTS produced were tested with Xpert MTB/RIF and evaluated for accuracy and precision.Results: Validation testing across eight rounds of PT demonstrated that DTS are highly accurate, achieving an average of 96.8% concordance with the Xpert MTB/RIF results from the original mycobacterial strains. Dried tube specimen testing was also precise, with cycle threshold standard deviations below two cycles when inherent test cartridge variability was low.Conclusion: Dried tube specimens can be produced using equipment already present in tuberculosis culture laboratories, making Xpert MTB/RIF PT scale-up more feasible in resource-limited settings. Use of DTS may fill the gap in tuberculosis laboratory access to external quality assessment, which is an essential component of a comprehensive continuous quality improvement programme. | |
| Publisher | AOSIS | |
| Date | 2020-09-29 | |
| Identifier | 10.4102/ajlm.v9i1.1166 | |
| Source | African Journal of Laboratory Medicine; Vol 9, No 1 (2020); 8 pages 2225-2010 2225-2002 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ajlmonline.org/index.php/ajlm/article/view/1166/1667
https://ajlmonline.org/index.php/ajlm/article/view/1166/1666
https://ajlmonline.org/index.php/ajlm/article/view/1166/1668
https://ajlmonline.org/index.php/ajlm/article/view/1166/1665
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