Study on the prevalence and genetic diversity of Eimeria species from broilers and free-range chickens in KwaZulu-Natal province, South Africa
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | Study on the prevalence and genetic diversity of Eimeria species from broilers and free-range chickens in KwaZulu-Natal province, South Africa | |
| Creator | Fatoba, Abiodun J. Zishiri, Oliver T. Blake, Damer P. Peters, Sunday O. Lebepe, Jeffrey Mukaratirwa, Samson Adeleke, Matthew A. | |
| Description | This study was conducted from January to October 2018 with the objective to determine the prevalence and genetic diversity of Eimeria species in broiler and free-range chickens in KwaZulu-Natal province, South Africa. A total of 342 faecal samples were collected from 12 randomly selected healthy broiler chicken farms and 40 free-range chickens from 10 different locations. Faecal samples were screened for the presence of Eimeria oocysts using a standard flotation method. The species of Eimeria isolates were confirmed by amplification of the internal transcribed spacer 1 (ITS-1) partial region and sequences analysis. Among broiler and free-ranging chickens, 19 out of 41 pens (46.3%) and 25 out of 42 faecal samples (59.5%) were positive for Eimeria infection. Molecular detection revealed the following species: Eimeria maxima, Eimeria tenella, Eimeria acervulina, Eimeria brunetti and Eimeria mitis in all the samples screened. Similarly, polymerase chain reaction assays specific for three cryptic Eimeria operational taxonomic units were negative for all the samples. Phylogenetic analysis of the ITS-1 sequences supported species identity with the greatest variation detected for E. mitis. This study provides information on the range and identity of Eimeria species, and their genetic relatedness, circulating in commercially reared broilers and free-ranging chickens from different locations in KwaZulu-Natal province. | |
| Publisher | AOSIS | |
| Date | 2020-09-17 | |
| Identifier | 10.4102/ojvr.v87i1.1837 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 87, No 1 (2020); 10 pages 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/1837/2113
https://ojvr.org/index.php/ojvr/article/view/1837/2112
https://ojvr.org/index.php/ojvr/article/view/1837/2114
https://ojvr.org/index.php/ojvr/article/view/1837/2111
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