In vitro propagation and genome sequencing of three ‘atypical’ Ehrlichia ruminantium isolates

Onderstepoort Journal of Veterinary Research

Field Value
Title In vitro propagation and genome sequencing of three ‘atypical’ Ehrlichia ruminantium isolates
Creator Liebenberg, Junita Steyn, Helena C. Josemans, Antoinette I. Faber, Erika Zweygarth, Erich
Subject — Ehrlichia ruminantium; heartwater; in vitro isolation; tick cell line; comparative genomics
Description Three isolates of Ehrlichia ruminantium (Kümm 2, Omatjenne and Riverside), the causative agent of heartwater in domestic ruminants, were isolated in Ixodes scapularis (IDE8) tick cell cultures using the leukocyte fraction of infected sheep blood. All stocks were successfully propagated in IDE8 cells, whereas initiation attempts using endothelial cell cultures were unsuccessful. Therefore, the new technique should be included in any attempt to isolate field strains of E. ruminantium to enhance the probability of getting E. ruminantium isolates which might not be initiated in endothelial cells. Draft genome sequences of all three isolates were generated and compared with published genomes. The data confirmed previous phylogenetic studies that these three isolates are genetically very close to each other, but distinct from previously characterised E. ruminantium isolates. Genome comparisons indicated that the gene content and genomic synteny were highly conserved, with the exception of the membrane protein families. These findings expand our understanding of the genetic diversity of E. ruminantium and confirm the distinct phenotypic and genetic characteristics shared by these three isolates.
Publisher AOSIS
Contributor Prof. Katherine M. Kocan, Oklahoma State University, Stillwater, OK, USA Gauteng Department of Agriculture and Rural Development, South Africa
Date 2020-06-24
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ojvr.v87i1.1769
Source Onderstepoort Journal of Veterinary Research; Vol 87, No 1 (2020); 14 pages 2219-0635 0030-2465
Language eng
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Rights Copyright (c) 2020 Junita Liebenberg, Helena C. Steyn, Antoinette I. Josemans, Erika Faber, Erich Zweygarth