Multilocus sequence typing of azole-resistant Candida auris strains, South Africa
Southern African Journal of Infectious Diseases
| Field | Value | |
| Title | Multilocus sequence typing of azole-resistant Candida auris strains, South Africa | |
| Creator | Magobo, Rindidzani Mhlanga, Mabatho Corcoran, Craig Govender, Nelesh P. | |
| Description | Background: Candida auris is an emerging multidrug-resistant fungal pathogen associated with high mortality.Methods: We investigated the genetic relatedness of clinical C. auris isolates from patients admitted to either public- or private-sector hospitals, which were submitted to a reference laboratory from 2012 to 2015. Patient demographics and clinical details were recorded. We performed antifungal susceptibility testing, sequencing of the hotspot 1 and 2 regions of the FKS1 and FKS2 genes for all isolates with an echinocandin minimum inhibitory concentration (MIC) of ≥1 µg/mL and cluster analysis using multilocus sequence typing.Results: Eighty-five isolates were confirmed as C. auris. The median patient age was 59 years [inter-quartile range (IQR): 48–68 years], with male patients accounting for 68% of cases. Specimen types included urine (29%), blood (27%), central venous catheter tips (25%), irrigation fluid (7%), tissue (5%), respiratory tract specimens (4%) and other (3%). Ninety-seven per cent of isolates were resistant to fluconazole, 7% were resistant to both fluconazole and voriconazole, 8% were resistant to both fluconazole and echinocandins (considered multidrug resistant) and all were susceptible to amphotericin B. Of the 15 randomly selected fluconazole-resistant isolates, 14 isolates had an isavuconazole MIC ≤ 1 µg/mL. No FKS mutations were detected. Multilocus sequence typing (MLST) analysis grouped isolates into two clusters: cluster 1 and cluster 2 comprising 83 and 2 isolates, respectively.Conclusions: Azole-resistant C. auris strains circulating in South African hospitals were related by MLST, but the possibility of nosocomial transmission should be explored using a more discriminatory technique, for example, whole genome sequencing. | |
| Publisher | AOSIS Publishing | |
| Date | 2020-03-23 | |
| Identifier | 10.4102/sajid.v35i1.116 | |
| Source | Southern African Journal of Infectious Diseases; Vol 35, No 1 (2020); 7 pages 2313-1810 2312-0053 | |
| Language | eng | |
| Relation |
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https://sajid.co.za/index.php/sajid/article/view/116/247
https://sajid.co.za/index.php/sajid/article/view/116/246
https://sajid.co.za/index.php/sajid/article/view/116/248
https://sajid.co.za/index.php/sajid/article/view/116/245
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