The use of aged stool specimens for the detection of rotavirus
Southern African Journal of Infectious Diseases
| Field | Value | |
| Title | The use of aged stool specimens for the detection of rotavirus | |
| Creator | de Bruyn, Karen Theron, Elizabeth M.C. Dewar, John B. Hendrick, Richard M. | |
| Description | Background: Rotavirus is considered worldwide as one of the most important viral gastrointestinal infections, resulting in potentially life-threatening diarrhoea and death in children under the age of 5 years. Rotavirus can survive and remain infectious for long periods outside of the human body and can be easily transmitted via environmental surfaces.Method: Stool specimens that had been collected and stored since 2010/2011 at 2°C – 8°C instead of −20°C or −80°C were analysed to determine the viability of rotavirus in these specimens after 6 years of improper storage. The specimens were analysed using simple enzyme immunoassay (EIA) methods from two different suppliers at different times throughout the period (2012–2017).Results: The analysis showed similar detection results for the two EIA kits.Conclusion: The rotavirus can be detected after several years of incorrect storage with EIA kits. | |
| Publisher | AOSIS Publishing | |
| Date | 2020-03-09 | |
| Identifier | 10.4102/sajid.v35i1.113 | |
| Source | Southern African Journal of Infectious Diseases; Vol 35, No 1 (2020); 3 pages 2313-1810 2312-0053 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://sajid.co.za/index.php/sajid/article/view/113/238
https://sajid.co.za/index.php/sajid/article/view/113/237
https://sajid.co.za/index.php/sajid/article/view/113/239
https://sajid.co.za/index.php/sajid/article/view/113/236
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