Antimicrobial usage in pig production: Effects on Escherichia coli virulence profiles and antimicrobial resistance

Onderstepoort Journal of Veterinary Research

Field Value
Title Antimicrobial usage in pig production: Effects on Escherichia coli virulence profiles and antimicrobial resistance
Creator Abubakar, Rukayya H. Madoroba, Evelyn Adebowale, Oluwawemimo Fasanmi, Olubunmi G. Fasina, Folorunso O.
Subject Microbiology antimicrobial; Escherichia coli; microbial drug resistance; virulence
Description Antimicrobials (AM) are used for growth promotion and therapy in pig production. Its misuse has led to the development of resistant organisms. We evaluated Escherichia coli virulence genes, and compared phenotypic–genotypic antimicrobial resistance (AMR) patterns of faecal E. coli from pigs receiving routine farm treatment without antimicrobial agents against pigs treated routinely with AM over 70 days. Recovered E. coli were tested for AMR using disk diffusion and polymerase chain reaction. Virulence genes were detected in 24.8% of isolates from antimicrobial group and 43.5% from non-antimicrobial group (p = 0.002). The proportion of virulence genes heat-stable enterotoxins a b (STa, STb), enteroaggregative heat stable enterotoxin 1 [EAST1] and Shiga toxin type 2e [Stx2e]) were 18.1%, 0.0%, 78.7% and 3.0% for antimicrobial group and 14.8%, 8.5%, 85.1% and 12.7% for non-antimicrobial groups, respectively. Resistance to oxytetracycline was most common (p = 0.03) in samples collected between days 10 and 21. Resistance shifted to amoxicillin on days 56–70, and trimethoprim resistance was observed throughout. Seventeen phenotypic AMR combinations were observed and eight were multidrug resistant. At least one tetracycline resistance gene was found in 63.9% of the isolates. tet (A) (23.3%) was most common in the antimicrobial group, whereas tet (B) (43.5%) was prevalent in the non-antimicrobial group. Usage or non-usage of antimicrobial agents in growing pigs does not preclude virulence genes development and other complex factors may be involved as previously described. Heavily used AM correspond to the degree of resistance and tetracycline resistance genes were detected during the growth phase.
Publisher AOSIS
Contributor National Research Foundation Incentive Funding for Rated Researchers (IPRR) granted to Fasina FO (grant number: 103854).
Date 2019-10-31
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — Quasi-Experimental
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/ojvr.v86i1.1743
Source Onderstepoort Journal of Veterinary Research; Vol 86, No 1 (2019); 11 pages 2219-0635 0030-2465
Language eng
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Coverage South Africa Contenporary Porcine, Age, Farm management
Rights Copyright (c) 2019 Rukayya H. Abubakar, Evelyn Madoroba, Oluwawemimo Adebowale, Olubunmi G. Fasanmi, Folorunso O. Fasina