Horizontal transfer of OXA-23-carbapenemase-producing Acinetobacter species in intensive care units at an academic complex hospital, Durban, KwaZulu-Natal, South Africa
Southern African Journal of Infectious Diseases
| Field | Value | |
| Title | Horizontal transfer of OXA-23-carbapenemase-producing Acinetobacter species in intensive care units at an academic complex hospital, Durban, KwaZulu-Natal, South Africa | |
| Creator | Swe Swe-Han, Khine Pillay, Melendhran Schnugh, Desmond Mlisana, Koleka P. Baba, Kamaldeen Pillay, Manormoney | |
| Description | Introduction: Carbapenemase production is an important mechanism of carbapenem resistance in Acinetobacter species. This study investigated the presence of the carbapenem-hydrolysing class D β–lactamase- encoding genes, blaOXA-23 and blaOXA-58, and their association with the spread of multidrug-resistant (MDR) Acinetobacter species in intensive care units at an academic hospital. Method: Forty-four MDR Acinetobacter species were confirmed using VITEK®2 and Epsilometer tests. The blaOXA-23 and blaOXA-58 genes were detected by polymerase chain reaction (PCR) in twenty-four selected isolates. The blaOXA-23 amplicons were sequenced and compared to the GenBank database. Genotypic relatedness of isolates was determined by pulsed field gel electrophoresis (PFGE). Clinical and laboratory data were analysed. Results: Among the twenty-four isolates, eighteen were carbapenem resistant and six were sensitive. The blaOXA-23 gene, but not blaOXA-58, was detected in the eighteen resistant strains. The blaOXA-23 amplicons showed 100% identity with the GenBank database of blaOXA-23. The MICs of carbapenems against Acinetobacter species carrying the blaOXA-23 gene were 8 to 16 μg/ml. Genetic relatedness was evident among isolates of seven pairs from fourteen patients. Of these patients, twelve were in the same ICUs and two were adjacent to another ICU during the same hospitalisation period. Conclusion: The selected MDR Acinetobacter species carried the blaOXA-23 gene responsible for resistance to carbapenems, while molecular and clinical data analysis suggested horizontal transmission in ICUs. In addition, the PFGE typing of a diverse collection of MDR Acinetobacter species clones showed that isolates were related to no more than two patients, suggesting that no outbreak had occurred. | |
| Publisher | AOSIS Publishing | |
| Date | 2017-12-31 | |
| Identifier | 10.4102/sajid.v32i4.36 | |
| Source | Southern African Journal of Infectious Diseases; Vol 32, No 4 (2017); 119-126 2313-1810 2312-0053 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://sajid.co.za/index.php/sajid/article/view/36/30
|
|
ADVERTISEMENT
