The recovery and molecular identification of HAdV-D17 in raw sewage and mussel samples collected in the Eastern Cape province of South Africa

Southern African Journal of Infectious Diseases

 
 
Field Value
 
Title The recovery and molecular identification of HAdV-D17 in raw sewage and mussel samples collected in the Eastern Cape province of South Africa
 
Creator Vos, Hillary J. Knox, Caroline M.
 
Subject — Human adenovirus (HAdV); mussels; nested PCR; raw sewage
Description Human adenoviruses (HAdV) are a common cause of clinical infections in South Africa. However, there is a lack of information regarding the prevalence and molecular identification of this virus in the environment. The objective of this study was to investigate the recovery and molecular identification of HAdV in sewage and mussel samples. All samples were subjected to transmission electron microscopy, viral DNA extraction and nested PCR amplification using adenovirus-specific primers targeting a conserved region of the hexon gene. Amplicons were cloned and sequenced and BLAST analysis revealed a closest matched sequence (98% identity) belonging to HAdV-D17.
 
Publisher AOSIS Publishing
 
Contributor
Date 2018-03-31
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format application/pdf
Identifier 10.4102/sajid.v33i1.27
 
Source Southern African Journal of Infectious Diseases; Vol 33, No 1 (2018); 4-7 2313-1810 2312-0053
 
Language eng
 
Relation
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:

https://sajid.co.za/index.php/sajid/article/view/27/22
 
Coverage — — —
Rights Copyright (c) 2019 Hillary J. Vos, Caroline M. Knox https://creativecommons.org/licenses/by/4.0
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