Die meting van die suurstofbindingseienskappe van hemosianien met die hulp van ’n dunlaag optiese sel
Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie/South African Journal of Science and Technology
| Field | Value | |
| Title | Die meting van die suurstofbindingseienskappe van hemosianien met die hulp van ’n dunlaag optiese sel Measurement of the oxygen binding properties of haemocyanin with the aid of a thin-layer optical cell | |
| Creator | van Aardt, W. J. | |
| Description | Die meriete van die dunlaagmetode van Dolman en Gill om die suurstofbindingseienskappe van hemosianien te meet, is met hemosianien afkomstig van arthropode en molluske getoets. Spektrofotometries is bevind dat oksihemosianien van sowel arthropode as molluske 'n prominente absorpsiepiek tussen 335 nm en 345 nm besit. Die resultate toon dat in vergelyking met hemoglobien hemosianien 'n beter stabiliteit in 'n dunlaagkonfigurasie besit. By 35 °C verminder die aanvanklike absorbansiewaarde met minder as 2% na drie uur blootstelling. Vir hemoglobien by 35 °C is die absorbansievermindering byna 10%. By 'n hoëaffiniteit- hemosianien van byvoorbeeld Marisa cornuarietis (P₅₀ = 1,07 mmHg) is Dolman en Gill se metode ook beter as ander metodes, omrede 'n verdunningsklep die PO₂-waardes by hierdie lae suurstofspannings noukeuriger kan uitmeet. In hierdie studie word 'n stap vir stap uiteensetting van die werkswyse ook verskaf. Die berekeninge wat gebruik is, word met toepaslike werksvoorbeelde verduidelik. Hopelik sal hierdie meettegniek hierdeur wyer in die respirasiefisiologie bekend gestel word. The merits of the thin-layer method of Dolman and Gill to study the oxygen binding of haemocyanin were experimentally tested with haemocyanin obtained from arthropods and molluscs. The results show that, spectrophotometrically, oxyhaemocyanin from both arthropods and molluscs has a prominent absorption peak between 335 and 345 nm. Haemocyanin is more stable in the thin- layer preparation when compared with haemoglobin. At 35 °C a decrease in the initial absorbance value of less than 2% was found after three hours. For haemoglobin the initial value decreases nearly 10% during the same period. For a high-affinity haemocyanin such as Marisa cornuarietis (P⁵⁰ = 1,07 mmHg) the thin-layer method of Dolman and Gill (1978) shows better PO₂ resolution than other methods. The reason for this is that the dilution valve, which is absent in other thin-layer methods, intrinsically measures the resultant PO₂ values at very low tensions more accurately. In this study a step-by-step explanation of the technique is given. The calculations have been explained using real figures and examples. It is hoped that this detailed description will make this technique more readily available for use by respiratory physiologists. | |
| Publisher | AOSIS | |
| Date | 1992-07-09 | |
| Identifier | 10.4102/satnt.v11i2.529 | |
| Source | Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie; Vol 11, No 2 (1992); 62-68 Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie; Vol 11, No 2 (1992); 62-68 2222-4173 0254-3486 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://journals.satnt.aosis.co.za/index.php/satnt/article/view/529/1390
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