’n Plastiek Cartesiese duiker as respirometer
Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie/South African Journal of Science and Technology
Field | Value | |
Title | ’n Plastiek Cartesiese duiker as respirometer A plastic Cartesian diver as respirometer | |
Creator | van Aardt, W. J. | |
Description | Die werkbeginsels van die Cartesiese duiker as respirometer vir terrestriele mikro-artropode word beskryf. Die voor - en nadele van Perspex as termoplastiese duikermateriaal teenoor Pyrex - of Jena-glas word aangetoon. Met hehulp van figure en fisiese afmetings word die funksionele onderdele van die Perspex-duiker, met 'n ykkonstante van ongeveer 80 mikroliters, beskryf. Aanwysings word stapsgewys verskaf bv. hoe die duikeropstelling (flotasiehouers, manometer, glasverdeelstuk) wat in 'n spesiaal geboude waterbad gehuisves is, gebruik word om reproduseerbare metinge van die ewewigsdruk (ΔP) te maak van diere wat minder as 1 mg in massa is. Die berekeninge wat nodig is om die ykkonstante van elke Perspex-duiker vas te stel word uiteengesit. ’n Praktiese voorbeeld word gebruik om die suurstofverbruikerskoers (VO₂) van ’n mytsoort vanaf die ewewigsdrukbepaling te bereken. The working principles of the Cartesian diver as a respirometer for terrestrial micro-athropods are discussed. The advantages and disadvantages of Perspex as thermoplastic diver material are compared with those of Pyrex or Jena glass. With the aid of diagrammes on which physical dimensions are indicated, the functional parts of a Perspex diver with a diver constant of about 80 microliters are described. Stepwise instructions for the operation of the diver system (flotation vessel, manometer, glassmanifold) within the enclosed waterbath are presented to enable one to measure the equilibrium pressure (ΔP) of each individual animal with a mass less than 1 mg. The calculations used to determine the diver constant for each diver are fully described. Practical examples are used to enable one to calculate the oxygen consumption rate (VO₂) for a mite species from the equilibrium pressure measurement. | |
Publisher | AOSIS | |
Date | 1991-07-08 | |
Identifier | 10.4102/satnt.v10i4.509 | |
Source | Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie; Vol 10, No 4 (1991); 169-175 Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie; Vol 10, No 4 (1991); 169-175 2222-4173 0254-3486 | |
Language | eng | |
Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://journals.satnt.aosis.co.za/index.php/satnt/article/view/509/913
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