Ultrastrukturele kwantifisering van mikrotubuli in volledige selprofiele
Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie/South African Journal of Science and Technology
| Field | Value | |
| Title | Ultrastrukturele kwantifisering van mikrotubuli in volledige selprofiele Ultrastructural quantification of microtubules in complete cell profiles | |
| Creator | Crous, A. de Beer, A. M. | |
| Description | Die noodsaaklikheid van intakte funksionele mikrotubuli vir insulienvrystelling deur die B-selle van pankreaseilande is welbekend. In glukose gestimuleerde B-selle korreleer die hoeveelheid mikrotubuli in die sitoplasma direk met die kwantiteit van insulienvrystelling, en word mikrotubuli dus as ’n essensiële komponent van die effektorsisteem van hierdie selle beskou. The ultrastructural morphometric quantification of cytoplasmic microtubular distribution densities requires electron micrograph magnifications whereby only small cytoplasmic areas are included per negative. Consecutive electron micrographs can be composed in montages to attain complete profiles of high magnification cell sections. Conventional morphometric methods are not suitable for application on areas that may be as great or even greater than 1 m² in size. Modifications of conventional morphometric technology to facilitate investigations of this nature are described. Statistical comparison of results obtained with this method and a standard method reflect the soundness of the modifications. | |
| Publisher | AOSIS | |
| Date | 1991-07-08 | |
| Identifier | 10.4102/satnt.v10i4.510 | |
| Source | Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie; Vol 10, No 4 (1991); 176-178 Suid-Afrikaanse Tydskrif vir Natuurwetenskap en Tegnologie; Vol 10, No 4 (1991); 176-178 2222-4173 0254-3486 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://journals.satnt.aosis.co.za/index.php/satnt/article/view/510/914
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