Antimicrobial activity of stigmasterol from the stem bark of Neocarya macrophylla

Journal of Medicinal Plants for Economic Development

Field Value
Title Antimicrobial activity of stigmasterol from the stem bark of Neocarya macrophylla
Creator Yusuf, Amina J. Abdullahi, Musa I. Aleku, Godwin A. Ibrahim, Ilyasu A.A. Alebiosu, Celestina O. Yahaya, Maryam Adamu, Hajara W. Sanusi, Abdulrazaq Mailafiya, Maria M. Abubakar, Hassan
Subject Pharmaceutical Chemisry, Natural Product Chemistry Neocarya macrophylla; stigmasterol; antimicrobial
Description Background: Natural products play a significant role in human therapy. They represent a huge reservoir of bioactive chemical diversity and help in understanding the cellular pathways that are essential component of drug discovery process. Objective: This study was aimed at evaluating the antimicrobial activity of stigmasterol isolated from the stem bark of Neocarya macrophylla. Methods: Stigmasterol previously isolated from the stem bark of N. macrophylla was subjected to antimicrobial screening against methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci (VRE), S. aureus, Streptococcus faecalis, Escherichia coli, Salmonella typhimurium, Pseudomonas fluorescens, Klebsiella pneumoniae, Candida albicans and Candida krusei using agar diffusion and broth dilution methods. Results: Susceptibility test results showed that the compound (100 μg/mL) inhibited the growth of all the test organisms with mean zone of inhibition range from 23 mm to 30 mm except the VRE, S. typhi and K. pneumoniae. The activity of stigmasterol was compared with that of ciprofloxacin (5 μg/mL), the standard antibacterial drug, and fluconazole (5 μg/mL), the antifungal agent. The test compound displayed a broad-spectrum of activity, and in many cases exhibited comparable antibacterial activity when compared to ciprofloxacin. Interestingly, the compound also showed antifungal activity against Candida spp., affording comparable inhibitory effect as fluconazole. The minimum inhibitory concentration (MIC) and the minimum bactericidal/fungicidal concentration (MBC/MFC) of stigmasterol range from 6.25 μg/mL to 25 μg/mL and from 12.5 μg/mL to 50 μg/mL, respectively. Conclusion: These properties suggest that the isolated stigmasterol is a potent and broad-spectrum antibacterial and antifungal agent and as such may serve as a lead compound in the development of novel antimicrobial drugs.
Publisher AOSIS Publishing
Date 2018-03-19
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — qualitative research
Format text/html application/epub+zip application/xml application/pdf
Identifier 10.4102/jomped.v2i1.38
Source Journal of Medicinal Plants for Economic Development; Vol 2, No 1 (2018); 5 pages 2616-4809 2519-559X
Language eng
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Coverage Africa — phytochemistry
Rights Copyright (c) 2018 Amina J. Yusuf, M. I. Abdullahi, G. A. Aleku, I. A.A. Ibrahim, C. O. Alebiosu, M. Yahaya, H. W. Adamu, A. Sanusi, M. M. Mailafiya, H. Abubakar