Construction of an artificial recombinant bicistronic plasmid DNA vaccine against porcine rotavirus
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | Construction of an artificial recombinant bicistronic plasmid DNA vaccine against porcine rotavirus | |
| Creator | Cui, Tingting Xiong, Jun Wang, Yongzhi Wen, Xintian Huang, Xiaobo Huang, Yong Ma, Xiaoping Hu, Zhongkai Zhao, Qin Cao, Sanjie | |
| Description | The attenuated Salmonella typhimurium χ4550 strain was used to harbour a reconstructed bicistronic DNA vaccine against porcine rotavirus, which carried the rotavirus nonstructural protein 4 (NSP4) and VP7 genes simultaneously. Using a balanced lethal system, the kanamycin resistance gene of expressing eukaryotic plasmids pVAX1 and pVAXD were replaced by the aspartate β-semialdehyde dehydrogenase (asd) gene. The NSP4 cleavage product (259–525) of rotavirus OSU strain and VP7 full-length genes were amplified by reverse transcription polymerase chain reaction and then inserted into the eukaryotic single-expression plasmid, pVAX1-asd, and the eukaryotic dual-expression plasmid, pVAXD-asd, respectively. The recombinant plasmids pVAX1-asd-NSP4, pVAX1-asd-VP7 and pVAXD-asd-NSP4-VP7 were transformed into the attenuated S. typhimurium χ4550 strain by electrotransformation. An indirect immunofluorescence assay of the expressed COS-7 cell suggested that the recombinant S. typhimurium χ4550 strain was constructed successfully. The recombinant S. typhimurium χ4550 strain was orally administered to BALB/c mice. The group immunised with dual- expression plasmids produced a significantly higher level of serum Immunoglobulin G (IgG) and intestinal Immunoglobulin A (IgA) than the group immunised with single-expression plasmids. These results indicated that eukaryotic bicistronic plasmid DNA vaccines could be successfully constructed to enhance humoural, mucosal and cellular immune response against rotavirus infection. | |
| Publisher | AOSIS | |
| Date | 2013-03-04 | |
| Identifier | 10.4102/ojvr.v80i1.498 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 80, No 1 (2013); 8 pages 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/498/784
https://ojvr.org/index.php/ojvr/article/view/498/785
https://ojvr.org/index.php/ojvr/article/view/498/786
https://ojvr.org/index.php/ojvr/article/view/498/783
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