Construction and immunogenicity of a ∆apxIC/ompP2 mutant of Actinobacillus pleuropneumoniae and Haemophilus parasuis
Onderstepoort Journal of Veterinary Research
Field | Value | |
Title | Construction and immunogenicity of a ∆apxIC/ompP2 mutant of Actinobacillus pleuropneumoniae and Haemophilus parasuis | |
Creator | Liu, Qiong Gong, Yuheng Cao, Yuqin Wen, Xintian Huang, Xiaobo Yan, Qigui Huang, Yong Cao, Sanjie | |
Description | The apxIC genes of the Actinobacillus pleuropneumoniae serovar 5 (SC-1), encoding the ApxIactivating proteins, was deleted by a method involving sucrose counter-selection. In this study, a mutant strain of A. pleuropneumoniae (SC-1) was constructed and named DapxIC/ ompP2. The mutant strain contained foreign DNA in the deletion site of ompP2 gene of Haemophilus parasuis. It showed no haemolytic activity and lower virulence of cytotoxicity in mice compared with the parent strain, and its safety and immunogenicity were also evaluated in mice. The LD50 data shown that the mutant strain was attenuated 30-fold, compared with the parent strain (LD50 of the mutant strain and parent strain in mice were determined to be 1.0 × 107 CFU and 3.5 × 105 CFU respectively). The mutant strain that was attenuated could secrete inactivated ApxIA RTX toxins with complete antigenicity and could be used as a candidate live vaccine strain against infections of A. pleuropneumoniae and H. parasuis. | |
Publisher | AOSIS | |
Date | 2013-03-06 | |
Identifier | 10.4102/ojvr.v80i1.519 | |
Source | Onderstepoort Journal of Veterinary Research; Vol 80, No 1 (2013); 6 pages 2219-0635 0030-2465 | |
Language | eng | |
Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/519/796
https://ojvr.org/index.php/ojvr/article/view/519/797
https://ojvr.org/index.php/ojvr/article/view/519/798
https://ojvr.org/index.php/ojvr/article/view/519/795
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