Enhanced diagnosis of rabies and molecular evidence for the transboundary spread of the disease in Mozambique
Journal of the South African Veterinary Association
| Field | Value | |
| Title | Enhanced diagnosis of rabies and molecular evidence for the transboundary spread of the disease in Mozambique | |
| Creator | Coetzer, Andre Anahory, Iolanda Dias, Paula T. Sabeta, Claude T. Scott, Terence P. Markotter, Wanda Nel, Louis H. | |
| Description | Rabies is a neglected zoonotic disease with veterinary and public health significance, particularly in Africa and Asia. The current knowledge of the epidemiology of rabies in Mozambique is limited because of inadequate sample submission, constrained diagnostic capabilities and a lack of molecular epidemiological research. We wanted to consider the direct, rapid immunohistochemical test (DRIT) as an alternative to the direct fluorescent antibody (DFA) for rabies diagnosis at the diagnostic laboratory of the Central Veterinary Laboratory (CVL), Directorate of Animal Science, Maputo, Mozambique. Towards this aim, as a training exercise at the World Organisation for Animal Health (OIE) Rabies Reference Laboratory in South Africa, we performed the DRIT on 29 rabies samples from across Mozambique. With the use of the DRIT, we found 15 of the 29 samples (52%) to be negative. The DRIT-negative samples were retested by DFA at the OIE Rabies Reference Laboratory, as well as with an established real-time Polymerase chain reaction, confirming the DRIT-negative results. The DRIT-positive results (14/29) were retested with the DFA and subsequently amplified, sequenced and subjected to phylogenetic analyses, confirming the presence of rabies RNA. Molecular epidemiological analyses that included viruses from neighbouring countries suggested that rabies cycles within Mozambique might be implicated in multiple instances of cross-border transmission. In this regard, our study has provided new insights that should be helpful in informing the next steps required to better diagnose, control and hopefully eliminate rabies in Mozambique. | |
| Publisher | AOSIS | |
| Date | 2017-03-24 | |
| Identifier | 10.4102/jsava.v88i0.1397 | |
| Source | Journal of the South African Veterinary Association; Vol 88 (2017); 9 pages 2224-9435 1019-9128 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://jsava.co.za/index.php/jsava/article/view/1397/1866
https://jsava.co.za/index.php/jsava/article/view/1397/1865
https://jsava.co.za/index.php/jsava/article/view/1397/1867
https://jsava.co.za/index.php/jsava/article/view/1397/1863
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