Experimental phage therapy against haematogenous multi-drug resistant Staphylococcus aureus pneumonia in mice
African Journal of Laboratory Medicine
| Field | Value | |
| Title | Experimental phage therapy against haematogenous multi-drug resistant Staphylococcus aureus pneumonia in mice | |
| Creator | Ochieng' Oduor, Joseph M. Onkoba, Nyamongo Maloba, Fredrick Nyachieo, Atunga | |
| Description | Background: Community-acquired haematogenous Staphylococcus aureus pneumonia is a rare infection, though it can be acquired nosocomially. Currently, antibiotics used against S. aureus pneumonia have shown reduced efficacy. Thus, there is need for an alternative therapy against multidrug-resistant S. aureus (MDRSA) strains in the community.Objective: We sought to determine the efficacy of environmentally-obtained S. aureus lytic phage against haematogenous MDRSA pneumonia in mice.Methods: Phages and MDRSA were isolated from sewage samples collected within Nairobi County, Kenya. Isolated S. aureus bacteria were screened for resistance against ceftazidime, oxacillin, vancomycin, netilmicin, gentamicin, erythromycin, trimethroprim-sulfamethoxazole and cefuroxime. Thirty BALB/c mice aged six to eight weeks were randomly assigned into three groups: the MDRSA-infection group (n = 20), the phage-infection group (n = 5) and the non-infection group (n = 5). Mice were infected with either MDRSA or phage (108 CFU/mL) and treated after 72 hours with a single dose of clindamycin (8 mg/kg/bwt) or 108 PFU/mL of phage or a combination therapy (clindamycin and phage). The efficacy of phage, clindamycin or clindamycin with phage combination was determined using resolution of lung pathology and bacterial load in lung homogenates.Results: The viable MDRSA count was 0.5 ± 0.2 log10 CFU/gm in the phage-treated group, 4.4 ± 0.2 log10 CFU/gm in the clindamycin-treated group and 4.0 ± 0.2 log10 CFU/gm in the combination-treated group. The efficacy of phage therapy was significantly different from other therapeutic modes (p = 0 0.0001). Histology showed that the mice treated with phage did not develop pneumonia.Conclusion: Phage therapy is effective against haematogenous MDRSA infection. Thus, it can be explored as an alternative treatment method. | |
| Publisher | AOSIS | |
| Date | 2016-09-30 | |
| Identifier | 10.4102/ajlm.v5i1.435 | |
| Source | African Journal of Laboratory Medicine; Vol 5, No 1 (2016); 7 pages 2225-2010 2225-2002 | |
| Language | eng | |
| Relation |
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https://ajlmonline.org/index.php/ajlm/article/view/435/2364
https://ajlmonline.org/index.php/ajlm/article/view/435/2365
https://ajlmonline.org/index.php/ajlm/article/view/435/2366
https://ajlmonline.org/index.php/ajlm/article/view/435/2367
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