Molecular characterisation of rifampicin-resistant Mycobacterium tuberculosis strains from Malawi
African Journal of Laboratory Medicine
| Field | Value | |
| Title | Molecular characterisation of rifampicin-resistant Mycobacterium tuberculosis strains from Malawi | |
| Creator | Chikaonda, Tarsizio Ketseoglou, Irene Nguluwe, Nelson Krysiak, Robert Thengolose, Isaac Nyakwawa, Felix Rosenberg, Nora E. Stanley, Christopher Mpunga, James Hoffman, Irving F. Papathanasopoulos, Maria A. Hosseinipour, Mina Scott, Lesley Stevens, Wendy | |
| Description | Background: Availability and access to the detection of resistance to anti-tuberculosis drug sremains a significant challenge in Malawi due to limited diagnostic services. The Xpert® MTB/RIF can detect Mycobacterium tuberculosis and resistance to rifampicin in a single, rapid assay. Rifampicin-resistant M. tuberculosis has not been well studied in Malawi.Objectives: We aimed to determine mutations in the rifampicin resistance determining region (RRDR) of the rpoB gene of M. tuberculosis strains which were defined as resistant to rifampicin by the Xpert MTB/RIF assay.Methods: Rifampicin-resistant isolates from 43 adult patients (≥ 18 years) from various districts of Malawi were characterised for mutations in the RRDR (codons 507–533) of the rpoB gene by DNA sequencing.Results: Mutations were found in 37/43 (86%) of the resistant isolates in codons 511, 512, 513,516, 522, 526 and 531. The most common mutations were in codons 526 (38%), 531 (29.7%) and 516 (16.2%). Mutations were not found in 6/43 (14%) of the resistant isolates. No novel rpoB mutations other than those previously described were found among the rifampicin-resistant M. tuberculosis complex strains.Conclusion: This study is the first to characterise rifampicin resistance in Malawi. The chaintermination DNA sequencing employed in this study is a standard method for the determination of nucleotide sequences and can be used to confirm rifampicin resistance obtained using other assays, including the Xpert MTB/RIF. Further molecular cluster analysis, such as spoligotyping and DNA finger printing, is still required to determine transmission dynamics and the epidemiological link of the mutated strains. | |
| Publisher | AOSIS | |
| Date | 2017-03-31 | |
| Identifier | 10.4102/ajlm.v6i2.463 | |
| Source | African Journal of Laboratory Medicine; Vol 6, No 2 (2017); 7 pages 2225-2010 2225-2002 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ajlmonline.org/index.php/ajlm/article/view/463/797
https://ajlmonline.org/index.php/ajlm/article/view/463/796
https://ajlmonline.org/index.php/ajlm/article/view/463/798
https://ajlmonline.org/index.php/ajlm/article/view/463/782
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