The use of real-time polymerase chain reaction and an adenosine deaminase assay for diagnosing pleural tuberculosis
African Journal of Laboratory Medicine
| Field | Value | |
| Title | The use of real-time polymerase chain reaction and an adenosine deaminase assay for diagnosing pleural tuberculosis | |
| Creator | Molaudzi, Mulalo Molepo, Julitha | |
| Description | Background: The diagnosis of pleural tuberculosis remains a challenge, because the most widely used conventional diagnostic tools are unable to rapidly detect Mycobacterium tuberculosis in pleural fluid with sufficient sensitivity.Objectives: The aim of this study was to evaluate the usefulness of an adenosine deaminase assay and real-time polymerase chain reaction (qPCR) in diagnosing pleural tuberculosis.Methods: One hundred and five consecutive pleural fluid specimens collected between August 2008 and March 2009 were assessed. Among the 105 specimens, 50 (48%) were unconfirmed tuberculosis cases, 21 (20%) were confirmed tuberculosis cases and 34 (32%) were non-tuberculosis cases (controls). Real-time PCR was performed using the Light Cycler Mycobacterium detection kit according to the manufacturer‘s instructions (Roche Diagnostics). An adenosine deaminase assay was carried out using a commercial colorimetric assay kit as a user-defined method on a Beckman DxC 600 Synchron analyser.Results: The sensitivity of the qPCR was 67% and specificity was 100%. The sensitivity of the adenosine deaminase assay was 80% and specificity was 94%.Conclusion: The findings show that the adenosine deaminase assay had higher sensitivity than qPCR. Real-time PCR had 100% specificity, thus a combination of the two methods may be useful for the diagnosis of pleural tuberculosis. | |
| Publisher | AOSIS | |
| Date | 2019-08-28 | |
| Identifier | 10.4102/ajlm.v8i1.731 | |
| Source | African Journal of Laboratory Medicine; Vol 8, No 1 (2019); 5 pages 2225-2010 2225-2002 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ajlmonline.org/index.php/ajlm/article/view/731/1335
https://ajlmonline.org/index.php/ajlm/article/view/731/1334
https://ajlmonline.org/index.php/ajlm/article/view/731/1336
https://ajlmonline.org/index.php/ajlm/article/view/731/1333
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