Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay
African Journal of Laboratory Medicine
| Field | Value | |
| Title | Detection of the Janus kinase 2 V617F mutation using a locked nucleic-acid, real-time polymerase chain reaction assay | |
| Creator | Senamela, Tshiphiri Kock, Marleen Becker, Piet Potgieter, Joachim J.C. | |
| Description | The purpose of this study was to develop a real time polymerase chain reaction (PCR) assay for the detection of the JAK2 V617F mutation that could be used in diagnostic laboratories. Sanger sequencing and a newly developed locked nucleic-acid, real-time PCR assay were used to detect the JAK2 V617F mutation. There was 100% agreement between the sequencing and PCR analysis. Both assays were able to detect the mutation in all 24 of the 60 test specimens harbouring the mutation. | |
| Publisher | AOSIS | |
| Date | 2018-01-31 | |
| Identifier | 10.4102/ajlm.v7i1.658 | |
| Source | African Journal of Laboratory Medicine; Vol 7, No 1 (2018); 4 pages 2225-2010 2225-2002 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ajlmonline.org/index.php/ajlm/article/view/658/962
https://ajlmonline.org/index.php/ajlm/article/view/658/961
https://ajlmonline.org/index.php/ajlm/article/view/658/963
https://ajlmonline.org/index.php/ajlm/article/view/658/960
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