Heterogeneity of Campylobacter species isolated from serial stool specimens of Egyptian children using pulsed field gel electrophoresis

African Journal of Laboratory Medicine

Field Value
Title Heterogeneity of Campylobacter species isolated from serial stool specimens of Egyptian children using pulsed field gel electrophoresis
Creator El-Gendy, Atef M. Wasfy, Momtaz O. Mansour, Adel M. Oyofo, Buhari T. Yousry, Marwa M. Klena, John D.
Subject Microbiology / Molecular Biology, Campylobacter spp Heterogeneity; Campylobacter spp.; serial stool specimens; Egyptian; children; pulsed field gel electrophoresis
Description Background: The genus Campylobacter spp. is a common cause of human acute bacteria lenteritis and travellers’ diarrhoea worldwide.Objective: To determine whether multiple serial isolations of Campylobacter spp., when obtained from a single child, represented the same or a different organism.Methods: In a birth cohort study conducted in Egypt, numerous children showed serial isolations of Campylobacter spp. Of these, 13 children were selected from different households based on the successive isolation of six or more Campylobacter isolates from stool samples.Results: Eighty isolates were recovered and identified as either Campylobacter coli (n = 25) or Campylobacter jejuni (n = 55). Pulsed-field gel electrophoresis (PFGE) revealed the presence of 38 unique C. jejuni and 24 C. coli profiles at a similarity level of ≥ 90%. Although no seriallyidentical isolates were detected in six children, others demonstrated at least one identical couple of isolates; all identified serially between one to six weeks. Two children demonstrated 80% similar couples of isolates that appeared seven months apart. PFGE could be a useful tool for differentiating reinfection, relapse and convalescent excretion phases.Conclusion: Our data suggest that Campylobacter infection in children is a complex process; children are exposed to multiple species in endemic environments and strains of the same bacterium appear to be shed serially between one to six weeks after the first exposure. Isolates that persisted for longer periods were relatively less similar, as shown from the results of this study.
Publisher AOSIS
Contributor United States Naval Medical Research Unit No. 3, Cairo, Egypt (NAMRU-3).
Date 2013-07-26
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — Bacteriological culture, Pulsed-field gel electrophoresis, antimicrobial susceptibility Testing, PCR
Format text/html application/octet-stream text/xml application/pdf
Identifier 10.4102/ajlm.v2i1.34
Source African Journal of Laboratory Medicine; Vol 2, No 1 (2013); 9 pages 2225-2010 2225-2002
Language eng
Relation https://ajlmonline.org/index.php/ajlm/article/view/34/113 https://ajlmonline.org/index.php/ajlm/article/view/34/114 https://ajlmonline.org/index.php/ajlm/article/view/34/115 https://ajlmonline.org/index.php/ajlm/article/view/34/112
Coverage Egypt From January 2004 to April 2007 Clinical, stool, archived isolates
Rights Copyright (c) 2013 Atef M. El-Gendy, Momtaz O. Wasfy, Adel M. Mansour, Buhari T. Oyofo, Marwa M. Yousry, John D. Klena https://creativecommons.org/licenses/by/4.0