Record Details

Molecular monitoring of African swine fever virus using surveys targeted at adult Ornithodoros ticks : a re-evaluation of Mkuze Game Reserve, South Africa

Onderstepoort Journal of Veterinary Research


 
 
Field Value
 
Title Molecular monitoring of African swine fever virus using surveys targeted at adult Ornithodoros ticks : a re-evaluation of Mkuze Game Reserve, South Africa
 
Creator Arnot, L.F. du Toit, J.T. Bastos, A.D.S.
 
Subject — —
Description The Mkuze Game Reserve (MGR), in north-eastern KwaZulu-Natal Province, South Africa is an African swine fever virus (ASF) controlled area. In a survey conducted in 1978, ASF prevalence in warthogs and Ornithodoros ticks in MGR was determined to be 2 % and 0.06 %, respectively. These values, acknowledged as being unusually low compared to other East and southern African ASF-positive sylvatic-cycle host populations, have not been assessed since. The availability of a sensitive PCR-based virus detection method, developed specifically for the sylvatic tampan host, prompted a re-evaluation of ASF virus (ASFV) prevalence in MGR ticks. Of the 98 warthog burrows inspected for Ornithodoros presence, 59 (60.2 %) were found to contain tampans and tick sampling was significantly male-biased. Whilst gender sampling-bias is not unusual, the 27 % increase in infestation rate of warthog burrows since the 1978 survey is noteworthy as it anticipates a concomitant increase in ASFV prevalence, particularly in light of the high proportion (75 %) of adult ticks sampled. However, despite DNA integrity being confirmed by internal control amplification of the host 16S gene, PCR screening failed to detect ASFV. These results suggest that ASFV has either disappeared from MGR or if present, is localized, occurring at exceptionally low levels. Further extensive surveys are required to establish the ASFV status of sylvatic hosts in this controlled area.
 
Publisher AOSIS Publishing
 
Contributor
Date 2009-09-09
 
Type — —
Format application/pdf
Identifier 10.4102/ojvr.v76i4.22
 
Source Onderstepoort J Vet Res; Vol 76, No 4 (2009); 385-392
 
Language en
 
Coverage — — —
Rights Copyright information Ownership of copyright in terms of the Work remains with the authors. The authors retain the non-exclusive right to do anything they wish with the Work, provided attribution is given to the place and detail of original publication, as set out in the official citation of the Work published in the journal. The retained right specifically includes the right to post the Work on the authors’ or their institutions’ websites or institutional repository. Publication and user license The authors grant the title owner and the publisher an irrevocable license and first right and perpetual subsequent right to (a) publish, reproduce, distribute, display and store the Work in  any form/medium, (b) to translate the Work into other languages, create adaptations, summaries or extracts of the Work or other derivative works based on the Work and exercise all of the rights set forth in (a) above in such translations, adaptations, summaries, extracts and derivative works, (c) to license others to do any or all of the above, and (d) to register the Digital Object Identifier (DOI) for the Definitive Work. The authors acknowledge and accept the user licence under which the Work will  be published as set out in http://creativecommons.org/licenses/by/2.5/za/legalcode (Creative Commons Attribution License South Africa) The undersigned warrant that they have the authority to license these publication rights and that no portion of the copyright to the Work has been assigned or licensed previously to any other party. Disclaimer: The publisher, editors and title owner accept no responsibility for any statement made or opinion expressed by any other person in this Work. Consequently, they will not be liable for any loss or damage sustained by any reader as a result of his or her action upon any statement or opinion in this Work. In cases where a manuscript is NOT accepted for publication by the editorial board, the portions of this agreement regarding the publishing licensing shall be null and void and the authors will be free to submit this manuscript to any other publication for first publication. Our copyright policies are author-friendly and protect the rights of our authors and publishing partners.