Record Details

Detection and characterisation of papillomavirus in skin lesions of giraffe and sable antelope in South Africa

Journal of the South African Veterinary Association


 
 
Field Value
 
Title Detection and characterisation of papillomavirus in skin lesions of giraffe and sable antelope in South Africa
 
Creator van Dyk, E.; Department of Companion Animal Clinical Studies,
Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa. Bosman, A-M; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa. van Wilpe, E.; Section of Electron Microscopy, Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa. Williams, J. H.; Section of Pathology, Department of Paraclinical
Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa. Bengis, R. G.; National Department of Agriculture, PO Box 12,
Skukuza, Kruger National Park, 1350 South Africa. van Heerden, J.; Kimberley Veterinary Clinic, 16 Dalham Road, Kimberley, 8301 South Africa. Venter, E. H.; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 South Africa.
 
Subject — —
Description Papillomavirus was detected electron microscopically in cutaneous fibropapillomas of a giraffe (Giraffa camelopardalis) and a sable antelope (Hippotragus niger). The virus particles measured 45 nm in diameter. Histopathologically, the lesions showed histopathological features similar to those of equine sarcoid as well as positive immunoperoxidase-staining of tissue sections for papillomavirus antigen. Polymerase chain reaction (PCR) detected bovine papillomavirus (BPV) DNA. Bovine papillomavirus-1 was characterised by real-time PCR in the sable and giraffe, and cloning and sequencing of the PCR product revealed a similarity to BPV-1. As in the 1st giraffe, the lesions from a 2nd giraffe revealed locally malignant pleomorphism, possibly indicating the lesional end-point of papilloma infection. Neither virus particles nor positively staining papillomavirus antigen could be demonstrated in the 2nd giraffe but papillomavirus DNA was detected by real-time PCR which corresponded with BPV-1 and BPV-2.
 
Publisher AOSIS Publishing
 
Contributor
Date 2011-04-11
 
Type — —
Format application/pdf
Identifier 10.4102/jsava.v82i2.39
 
Source Journal of the South African Veterinary Association; Vol 82, No 2 (2011); 80-85
 
Language en
 
Coverage — — —
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