An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa

Journal of the South African Veterinary Association

 
 
Field Value
 
Title An evaluation of serological tests in the diagnosis of bovine brucellosis in naturally infected cattle in KwaZulu-Natal province in South Africa
 
Creator Chisi, Songelwayo L. Marageni, Yoanda Naidoo, Prebashni Zulu, Gloria Akol, George W. van Heerden, Henriette
 
Subject veterinary science; microbiology; diagnostics complement fixation test; indirect enzyme linked immuno absorbent assay; competitive enzyme linked immuno absorbent assay; Rose Bengal test; serum agglutination test; brucella abortus; diagnostic sensitivity; diagnostic specificity
Description The diagnostic sensitivity (DSe) of the Rose Bengal test (RBT), the complement fixation test (CFT), the serum agglutination test (SAT), the competitive enzyme-linked immunosorbent assay (cELISA) and the indirect ELISA (iELISA) were determined in naturally infected cattle in KwaZulu-Natal province of South Africa with known infectious status from culture (gold standard). Natural brucellosis infection status of animals was determined by culturing and identification of Brucella abortus biovar 1 from abomasal fluid, milk, hygroma fluid, lymph nodes or uterine discharges samples. The diagnostic specificity (DSp) of the tests mentioned above was determined using samples from known negative herds. There was no statistically significant difference between the tests in their ability to diagnose brucellosis. The RBT and iELISA had the highest DSe of 95.8%, whereas RBT and CFT had the highest DSp of 100%. In South African laboratories, the RBT and CFT serological tests are used, because of the cost efficacy of CFT when compared to the less labour intensive but more expensive iELISA.
 
Publisher AOSIS
 
Contributor Department of Agriculture and Rural Development, Province of KwaZulu Natal, South Africa
Date 2017-02-28
 
Type info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion — —
Format text/html application/epub+zip text/xml application/pdf
Identifier 10.4102/jsava.v88i0.1381
 
Source Journal of the South African Veterinary Association; Vol 88 (2017); 7 pages 2224-9435 1019-9128
 
Language eng
 
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https://jsava.co.za/index.php/jsava/article/view/1381/1858 https://jsava.co.za/index.php/jsava/article/view/1381/1857 https://jsava.co.za/index.php/jsava/article/view/1381/1859 https://jsava.co.za/index.php/jsava/article/view/1381/1849
 
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Rights Copyright (c) 2017 Songelwayo L. Chisi, Yoanda Marageni, Prebashni Naidoo, Gloria Zulu, George W. Akol, Henriette van Heerden https://creativecommons.org/licenses/by/4.0
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