Multiplex PCR for differentiating Ornithodoros tick vectors in South Africa: Proof of concept
Onderstepoort Journal of Veterinary Research
| Field | Value | |
| Title | Multiplex PCR for differentiating Ornithodoros tick vectors in South Africa: Proof of concept | |
| Creator | West, Susan van Emmenes, Juanita Boshoff, Carin | |
| Description | African swine fever (ASF) is a severe viral haemorrhagic disease affecting domestic pigs, maintained by ASF virus within an ancient sylvatic cycle involving Ornithodoros ticks that inhabit warthog burrows. This study aimed to develop and optimise a multiplex polymerase chain reaction (PCR) assay to differentiate three Ornithodoros species in South Africa – O. moubata, O. phacochoerus and O. waterbergensis, to enhance ASF vector identification and management. The final multiplex PCR effectively distinguished between species with specific amplicon sizes: 180 base pairs (bp) for O. moubata, 640 bp for O. phacochoerus and 1000 bp for O. waterbergensis.Contribution: This refined multiplex PCR method demonstrates proof of concept as a reliable and rapid tool for distinguishing Ornithodoros species and correlating them with their geographical origins. This assay, based on amplification size, provides crucial information about the distribution of these species, which could enhance ASF control efforts. | |
| Publisher | AOSIS | |
| Date | 2025-10-27 | |
| Identifier | 10.4102/ojvr.v92i1.2225 | |
| Source | Onderstepoort Journal of Veterinary Research; Vol 92, No 1 (2025); 8 pages 2219-0635 0030-2465 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://ojvr.org/index.php/ojvr/article/view/2225/2758
https://ojvr.org/index.php/ojvr/article/view/2225/2759
https://ojvr.org/index.php/ojvr/article/view/2225/2760
https://ojvr.org/index.php/ojvr/article/view/2225/2761
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