Determination of the minimum protective dose for bluetongue virus serotype 2 and 8 vaccines in sheep
Journal of the South African Veterinary Association
| Field | Value | |
| Title | Determination of the minimum protective dose for bluetongue virus serotype 2 and 8 vaccines in sheep | |
| Creator | Modumo, Jacob Venter, Estelle H. | |
| Description | Recent outbreaks of bluetongue virus (BTV) serotypes 2 and 8 in many European countries provided an opportunity to investigate the possibility of improving the safety of the modified live vaccines administered mainly in South Africa. Modified live vaccines (MLV) released at a titre of 5 x 104 PFU/mL, raised concerns and prompted the need to determine the minimum titre which will still be protective and also safe. The BTV serotypes 2 and 8 vaccines were produced at the following titres: 102 PFU/mL, 103 PFU/mL and 104 PFU/mL, and were injected into 24 sheep which were then monitored. Blood was collected on days 0, 3, 6, 9, 12, 15, 18, 21, 25, 28 and 4 months post vaccination, for seroconversion and viraemia studies. These sheep were later challenged at 4 months post vaccination using BTV infected cell culture material, they were then observed and bled and again tested for viraemia. There was no viraemia post vaccination, however, a febrile reaction did occur and seroconversion was demonstrated at low titres for both BTV 2 and 8. Although viraemia was demonstrated post challenge, sheep vaccinated with the low titre BTV 2 vaccine showed more than a 90% protection index at a lower titre of 103 PFU/mL, compared with BTV 8 that showed a protection index above 90% at all the titres used. It is recommended that for BTV 2 vaccine, sheep should be vaccinated at a titre of 103 PFU/mL and at a titre of 102 PFU/mL with BTV 8 vaccine. | |
| Publisher | AOSIS | |
| Date | 2012-08-03 | |
| Identifier | 10.4102/jsava.v83i1.17 | |
| Source | Journal of the South African Veterinary Association; Vol 83, No 1 (2012); 6 pages 2224-9435 1019-9128 | |
| Language | eng | |
| Relation |
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https://jsava.co.za/index.php/jsava/article/view/17/890
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https://jsava.co.za/index.php/jsava/article/view/17/891
https://jsava.co.za/index.php/jsava/article/view/17/119
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https://jsava.co.za/index.php/jsava/article/downloadSuppFile/17/115
https://jsava.co.za/index.php/jsava/article/downloadSuppFile/17/116
https://jsava.co.za/index.php/jsava/article/downloadSuppFile/17/117
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