Description of non-polio enteroviruses identified in two national surveillance programmes in South Africa
Southern African Journal of Infectious Diseases
| Field | Value | |
| Title | Description of non-polio enteroviruses identified in two national surveillance programmes in South Africa | |
| Creator | Howard, Wayne Savulescu, Dana Berrie, Leigh Puren, Adrian J. | |
| Description | Background: Human enteroviruses (EV) consist of 106 serotypes and four species: EV-A, EV-B, EV-C and EV-D. Enteroviruses cause clinical symptoms varying from severe to mild. Knowledge of EV burden in South Africa is limited, and as non-polio EV are important causes of acute flaccid paralysis (AFP) and meningitis, information on the circulating serotypes is vital.Methods: Between 2010 and 2012, a total of 832 stool and viral isolate specimens were obtained from two national surveillance programmes at the National Institute for Communicable Diseases: the Rotavirus Sentinel Surveillance Programme (RSSP) and the AFP surveillance programme. Real-time polymerase chain reaction and Sanger sequencing were performed to detect and serotype EV.Results: Non-polio EV were detected in 446 specimens, of which 308 were sequenced. Stool specimens yielded a greater variety of serotypes than viral cultures. EV-B viruses were predominant (58.44%), whilst EV-C viruses were detected in 31% of the specimens tested. South African prevalence for these viruses was higher than other countries, such as France with less than 2%, and Spain and the United States with less than 10%. The most common serotype detected was Enterovirus 99 (EV-C, 8.63%), which has not been reported in other regions.Conclusion: Direct sequencing from stool specimens yields a broader, more comprehensive description of EV infections compared to sequencing from viral cultures. Disease-associated serotypes were detected, but only in small numbers. This study provides a baseline for EV strain circulation; however, surveillance needs to be expanded to improve EV knowledge in South Africa. | |
| Publisher | AOSIS Publishing | |
| Date | 2020-12-15 | |
| Identifier | 10.4102/sajid.v35i1.196 | |
| Source | Southern African Journal of Infectious Diseases; Vol 35, No 1 (2020); 7 pages 2313-1810 2312-0053 | |
| Language | eng | |
| Relation |
The following web links (URLs) may trigger a file download or direct you to an alternative webpage to gain access to a publication file format of the published article:
https://sajid.co.za/index.php/sajid/article/view/196/462
https://sajid.co.za/index.php/sajid/article/view/196/461
https://sajid.co.za/index.php/sajid/article/view/196/463
https://sajid.co.za/index.php/sajid/article/view/196/464
|
|
ADVERTISEMENT
